Sarconesiopsis magellanica (Le Guillou) (Diptera: Calliphoridae) is a necrophagous fly that is important in both human and veterinary medicines. This insect has been registered in Colombia as a biological indicator in estimating post-mortem interval. Insect cell cultures are an important biotechnological tool for basic and applied studies, and cell cultures derived from S. magellanica embryonic tissues are described in this study. S. magellanica embryonated eggs were taken for tissue explants. These were seeded in L-15, Grace/L-15, Eagle MEM, MM, VP12, MM/VP12, and Schneider culture media. The morphological, cytogenetic, biochemical, and molecular characteristics of the cell cultures were examined. Cell growth was achieved in the L15, Grace/L15, and Schneider culture media, and the confluent monolayers were obtained 8, 10, and 19 days after the embryonated eggs were explanted. However, the Schneider medium was the most efficient to develop the subcultures, and 21 passages have been maintained. The cell morphology of the primary cell cultures was initially heterogeneous, but in the confluent monolayer and in the subcultures there was greater cell morphology uniformity, fibroblastoid types being predominant. Cultured cells had a chromosomal number of 12, and the karyotypic complement consisted of five pairs of somatic chromosomes and one sexual pair. The cell culture isozyme patterns of S. magellanica coincided with adult samples from the same species. The molecular analysis, using RAPD-PCR, demonstrated the authentication of the cell cultures of this fly and their differentiation from other cultures derived from two sand flies species. This cell line is a new in vitro model that will be used in biomedical and biotechnological studies.
O presente trabalho teve como principal objetivo compreender alguns fatores que influenciam a sugestionabilidade interrogativa em pessoas idosas. Neste âmbito, procurou-se analisar a existência de diferenças na sugestionabilidade interrogativa em função da idade, bem como averiguar a sua relação com variáveis cognitivas e de ansiedade. A amostra incluiu 52 adultos idosos (78-83 anos), e igual número de adultos mais novos entre 42 e 52 anos. Utilizaram-se os seguintes instrumentos de avaliação: Escala de Sugestionabilidade de Gudjonsson 1 (GSS 1), Mini Mental State Examination, teste de Fluência Verbal de Categorias, subtestes Vocabulário e Memória de Dígitos da Escala de Inteligência para Adultos de Wechsler-III, Inventário de Ansiedade Estado-Traço para Adultos e Matrizes de Raven. Entre os grupos verificaram-se diferenças estatisticamente significativas nos resultados Cedência 1 e Mudança da GSS 1, Fluência Verbal, Memória de Dígitos e Ansiedade. No que respeita a valores de correlação moderados entre sugestionabilidade interrogativa e medidas cognitivas e de ansiedade, estes somente foram registados entre Sugestionabilidade Total e tempo de realização nas Matrizes de Raven, em ambos os grupos etários. Observou-se ainda que o conhecimento prévio, por parte de vários participantes, da entrevistadora que administrou a GSS 1 pode influenciar os resultados obtidos nesta escala. Os vários resultados são discutidos considerando a sua relevância forense.
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