SignificanceDespite being one of the most important foodstuffs consumed in the modern world, the origins of bread are still largely unknown. Here we report the earliest empirical evidence for the preparation of bread-like products by Natufian hunter-gatherers, 4,000 years before the emergence of the Neolithic agricultural way of life. The discovery of charred food remains has allowed for the reconstruction of the chaîne opératoire for the early production of bread-like products. Our results suggest the use of the wild ancestors of domesticated cereals (e.g. wild einkorn) and club-rush tubers to produce flat bread-like products. Cereal-based meals such as bread probably become staples when Neolithic farmers started to rely on the cultivation of domesticated cereal species for their subsistence.

‘Neolithization’ pathway refers to the development of adaptations that characterized subsequent Neolithic life, sedentary occupations, and agriculture. In the Levant, the origins of these human behaviors are widely argued to have emerged during the Early Epipaleolithic (ca. 23 ka cal BP). Consequently, there has been a pre-occupation with identifying and modeling the dietary shift to cereal and grains during this period, which is considered to have been a key development that facilitated increasing sedentism and, eventually, agriculture. Yet, direct evidence of plant use in the form of macrobotanical remains is extremely limited at Epipaleolithic sites and the expected ‘Neolithization’ pathway has not been robustly demonstrated. However, new direct microbotanical phytolith evidence from the large aggregation site of Kharaneh IV, in the Azraq Basin, suggests that increasingly settled occupation was not the result of wild grass and cereal use, but rather the result of a typical hunter-gatherer balance, based on the use of mostly reliable resources supplemented by some risky resources. Moreover, and illustrating this balance, the direct botanical evidence emphases the importance of the wetlands as an under-recognized reliable plant resource. Significantly, the use of these reliable wetland plant resources at Kharaneh IV represents an unexpected ‘Neolithization’ pathway.

The mosaic ecology of the Late Pleistocene Levant has been referred to by many authors investigating Epipaleolithic use of wild cereals as a prelude to cultivation, but it has rarely been the focus of studies investigating hunter-gatherer adaptations and diverse wild plant collection strategies. Partly, this was due to poor preservation of macro-botanical remains at Epipaleolithic sites with the exception of Ohalo II. Our analyses of 100 phytolith samples from four sites in the eastern Levant, compared with phytoliths from Ohalo II, reveal evidence for how foragers used the unique local ecological opportunities in these regions. Abundant phytoliths from reeds and sedges indicate the critical importance of wetlands in hunter-gatherer subsistence strategies. Wild cereals and grasses point to steppe and parkland exploitation, and woody plants indicate use of woodlands. From the Late Upper Paleolithic through the Middle Epipaleolithic our evidence

Epipaleolithic hunter-gatherers are often interpreted as playing an important role in the development of early cereal cultivation and subsequent farming economies in the Levant. This focus has come at the expense of understanding these people as resilient foragers who exploited a range of changing micro habitats through the Last Glacial Maximum. New phytolith data from Ohalo II seek to redress this. Ohalo II has the most comprehensive and important macrobotanical assemblage in Southwest Asia for the entire Epipaleolithic period. Here we present a phytolith investigation of 28 sediment samples to make three key contributions. First, by comparing the phytolith assemblage to a sample of the macrobotanical assemblage, we provide a baseline to help inform the interpretation of phytolith assemblages at other sites in Southwest Asia. Second, we highlight patterns of plant use at the site. We identify the importance of wetland plant resources to hut construction and provide evidence that supports previous work suggesting that grass and cereal processing may have been a largely “indoor” activity. Finally, drawing on ethnographic data from the American Great Basin, we reevaluate the significance of wetland plant resources for Epipaleolithic hunter-gatherers and argue that the wetland-centered lifeway at Ohalo II represents a wider Levantine adaptive strategy.