The number of obese older adults is on the rise, but data about proper treatment of obesity in the elderly is controversial. The present study was designed to investigate the effectiveness and consequences of partial subcutaneous lipectomy, as a rapid medical intervention against increased accumulation of body fat, in adult obese rats. The study was conducted on adult (9-12 months) female rats, in which obesity was induced by bilateral surgical ovariectomy. They were randomized into two main groups: short term (5 weeks) and long term (10 weeks). Both groups were subdivided into control, ovariectomized (OVX) and ovariectomized lipectomized groups. Body weight (BW) was measured and body mass index (BMI) calculated. Fasting blood glucose, lipid profile and plasma levels of total proteins, albumin, liver enzymes, malondialdehyde (MDA), leptin and adiponectin were determined. The content of both blood and hepatic tissue of reduced glutathione was estimated. In addition, histological study of the liver, aorta and perirenal fat was performed. Compared to controls, OVX rats showed significant increase in BW, BMI and plasma levels of liver enzymes, MDA and leptin. Histological study revealed vacuolated ballooned hepatocytes and enlarged irregular visceral adipocytes with atherosclerotic changes in the wall of aorta. Following subcutaneous lipectomy, rats exhibited significant fasting hyperglycaemia, dyslipidaemia, lowered plasma albumin and disturbed redox state with aggravation of the histological changes. The findings indicate that although subcutaneous lipectomy appears to be effective in combating obesity in older females, it has unfavourable effects on both metabolic and hepatic functions.
Background: Chronic kidney disease (CKD) is characterized by impaired kidney function, progressive kidney damage, unbalanced gut microbiota and disrupted intestinal mucosal barrier function. The damaged intestinal barrier functions mediated mostly by urea, allows influx of toxic products such as indoxyl sulphate that cause systemic inflammation. Activated charcoal is a universal antidote for the majority of poisons. Activated charcoal was suggested as a supplementary treatment for patients with CKD to remove waste products such as urea, indoxyl sulphate and other toxins .Objectives: This study was designed to investigate the possible role of activated charcoal in limiting the influx of intestinal bacterial toxins to systemic circulation to limit progression of CKD in albino rats.Material and Methods: Forty male white albino rats were divided into 4 equal groups. Sham operated control group (Group I): rats in this group were subjected to all steps of 5/6th nephrectomy except for kidney removal and sacrificed after 6 weeks, 5/6th nephrectomised group (Group II): were subjected to 5/6th nephrectomy operation, early charcoal treated 5/6th nephrectomised group (Group III ): were subjected to 5/6th nephrectomy operation and charcoal treatment (4g/kg/day) started immediately after operation for 6 weeks, and late charcoal treated 5/6th nephrectomised group (Group IV): were subjected to 5/6th nephrectomy operation and charcoal treatment started 2 weeks after operation continued for 4 weeks .Body weight and arterial blood pressure were measured before scarification after 6 weeks. Level of creatinine, urea, indoxyl sulphate and C-reactive protein were determined in the serum. Histological studies of pieces of terminal ileum and colon stained hematoxylin and eosin were done. Renal fibrosis index was assessed in remnant kidney stained with Masson Trichome .Results: Group II had elevated serum level of urea, creatinine, indoxyl sulfate and C-reactive protein, colonic erosions and renal fibrosis compared to control group. Group III showed decreased level of serum creatinine, urea, indoxyl sulphate and C-reactive protein with partial restoration of the colonic mucosal integrity and reduction in renal fibrosis. Group IV had altered serum creatinine, urea and C-reactive protein but not serum indoxyl sulphate. Arterial blood pressure elevated in all studied groups compared to control group and was not affected by charcoal administration. Conclusion:Activated charcoal has the ability to limit progression of CKD and the fibrotic changes in the kidney as well as to limit the associated intestinal barrier disruption, and the early therapy was more significant compared to late interference .
Purpose The deleterious effect of visceral adipose tissue accumulation is well known. However, the recent trend in liposuction is mal-directed toward easily accessible subcutaneous fat for the purpose of body shaping. The aim of the present study is to probe the metabolic effects of subcutaneous abdominal adipose tissue lipectomy in ovariectomized obese rats as well as the role of adipokines in these changes. Methods The study was conducted on young female rats randomized into two main groups according to the duration of the experiment, namely, 5-week and 10-week. Both groups were subdivided as follows: sham-operated, ovariectomized, and ovariectomized lipectomized rat groups. The rats underwent measurement of body weight (BW) and determination of body mass index (BMI). Fasting blood glucose, lipid profile, liver function, plasma malondialdehyde, leptin, and adiponectin were estimated, and the content of both blood and hepatic tissue of reduced glutathione was assessed. In addition, histological study of the liver, aorta, and perirenal fat of all rat groups was performed. Results Ovariectomy-induced obesity is marked by a significant increase in BW and BMI. Following subcutaneous lipectomy, the rats exhibited significant weight gain accompanied by fasting hyperglycemia, dyslipidemia, deterioration of synthetic function of the liver, and disturbed oxidant/antioxidant status. Histological examination revealed fatty infiltration of aortic and hepatic tissues. Conclusion Despite the immediate positive effect of subcutaneous lipectomy for weight loss and/or body shaping, multiple delayed hazards follow the procedure, which should be carefully considered.
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