The plant world is an excellent source of active ingredients, which gives it significant antibacterial activity, often sought after in alternative medicine and the food industry for food preservation. Our work focuses on the study of essential oil extraction and study of the biological activities of a medicinal plant, Urtica dioica (Urticaceae). The phytochemical screening study of the aerial part of the plant confirmed the presence of alkaloids, tannins, saponosides, flavonoids. And the absence of coumarins and volatile oil. The results obtained show that the water content of the studied plants was observed with 13.74%. The extraction yield in methanol-water mixture is low compared to that of water which is 20.8%. The results obtained for total polyphenol contents (mg EAG/g RS) in the two extracts are of the order of: 26.02(EM); 19.26 (AQE). The flavonoid content is around 86.43 (EAq); 10.26 (EM). Condensed tannin content is around 22.29 (EAq); 26.17 (EM).The percentage inhibition of the DPPH radical only reached 05% inhibition with the aqueous extract and even for the methanolic extract the activity did not exceed 19% inhibition of the DPPH radical. The antimicrobial activity was determined on four bacterial strains, according to the disc diffusion method, the four strains studied show sensitivity towards plant extracts, the results indicate that the two extracts have a good antimicrobial activity against the strains tested (Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Proteus mirabilis) and which act differently on the strains tested.
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