Estrus synchronization is a valuable management method in enhancing reproductive efficiency in small ruminants. Sheep is a seasonally polyestrus animal with spontaneous estrus and ovulation during periods of short daylight (Bartlewski et al., 2011). Obviously, restricting reproduction to the breeding season decreases individual productivity and profitability, especially in single-bearing breeds.Using estrus induction protocols during non-breeding season can solve this problem and raise the number of lamb per individual to three every 2 years.Introducing a novel ram to anestrus ewes can trigger an ovulatory LH surge through a series of neuroendocrine events within the hypothalamus and pituitary gland (Martin et al., 1980). However,
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