Regardless of all efforts to guarantee safety of blood, hepatitis B residual risk is the highest among transfusiontransmitted diseases [1]. HBV DNA was detected in 3.3% of blood donors negative for hepatitis B surface antigen (HbsAg) and hepatitis B surface antibody (HBsAb) but positive for anti-HBc [2].The frequency of HBsAg in Egyptian blood donors is 4.3% [3]. There are no available data about the frequency of either hepatitis B core antibody (HBcAb) or HBsAb in Egyptian blood donors. In order to evaluate the role of HBcAb in blood screening for HBV infection, 150 blood donors (negative for HBsAg, HBsAb, hepatitis C virus antibody, and human immunodeficiency virus) were tested for HBcAb (total). In the anti-HBc-positive samples (n = 20), HBV-DNA polymerase chain reaction (PCR) assay tests were performed. HBV-DNA was detected in only 2 (10%) out of the 20 HBcAb-positive samples. Isolated anti-HBc that tested negative for HBV DNA was detected in 18 (12%) of the 150 samples that tested negative for HBsAg.Isolated anti-HBc was reported in 2% of the 6,035 consecutive Saudi blood donors [4]. In a single Brazilian study, it was 10% [5]. In our institution, however, the prevalence of isolated HBcAb in tested samples was 13.3%. Isolated anti-HBc serologic profile may be associated with occult chronic HBV infection with undetectable HBsAg, remote HBV infection with loss of measurable HBsAb, passive transfer of anti-HBc, nonspecific crossreacting antibody, or the period when HBsAg has disappeared and anti-HBs has not been detected. The higher prevalence of isolated anti-HBc in our study may be related to the absence of donor selection.Molecular methods have demonstrated the presence of the virus in patients with HBcAb alone with frequencies of 0.8% and 12.2% in a Brazilian [6] and an Iranian [7] study, respectively. In our study, the overall prevalence of HBV-DNA in healthy blood donors was 10% among isolated anti-HBc-positive individuals. Such wide discrepancy, not completely understood, could result from virological, immunological, methodological, or epidemiological factors. The detection of HBV DNA by PCR in these individuals rules out the possibility of false positive results and remote HBV infection. At the same time, it confirms the presence of occult HBV infection.Individuals with isolated anti-HBc who tested negative for HBV DNA constituted 12% of our tested populations. These subjects may have remote infection, false positive result, or represent an immunological window period. We have tested subjects for total HBcAb rather than IgM and IgG isotypes. Therefore, the differentiation between acute and chronic HBV infection was difficult.The exclusion of all anti-HBc positive blood donors leads to unnecessary blood shortage in blood banks (12%