The present experiment was conducted to establish an efficient in vitro regeneration protocol for Alocasia amazonica through indirect organogenesis from young leaf segment. For callus induction, leaf explants were inoculated on MS medium containing different concentrations of 2,4-D. Highest callus induction frequency (90%) was recorded on MS medium supplemented with 4.0 mg/l 2,4-D. Remarkable results on indirect shoot organogenesis and multiplication (86.67%) with maximum shoot number per unit callus (11.64 ± 0.37) and shoot length (10.13 ± 0.24 cm) were observed when the leaf derived callus were transferred on MS medium fortified with 1.5 mg/l BAP and 0.5 mg/l NAA. Additionally, the incorporation of 10% coconut water with the medium showed satisfactory shoot growth and development. The best response towards root induction (85%) was achieved on ½ MS medium fortified with 3.0 mg/l IBA, 2.0 mg/l IAA and 2.0 mg/l NAA with an average 22.20 ± 0.87 roots per unit shoot. Well rooted plantlets were successfully acclimatized to the soil where the survival rate was 90%. Plant Tissue Cult. & Biotech. 32(1): 13-20, 2022 (June)
An experiment was performed to establish a suitable regeneration protocol for the rapid propagation of Piper nigrum L. using nodal segments and leaf tissues as explants through direct and indirect organogenesis. After surface sterilization, several types of explant were inoculated onto gelled MS medium containing various concentrations and combinations of growth regulators for callus and direct shoot induction. The highest callus induction frequency was 92% and 84% in the case of leaf tissues and nodal segments, respectively, in gelled MS medium containing 2.0 mg/l 2,4-D. Multiple shoots (6.43±0.35 shoots per unit callus) were obtained when the calli from both explant types were cultured on MS medium containing 1.0 mg/l BAP. Nodal segments showed the best result (85%) in terms of direct shoot induction in MS medium supplemented with 1.5 mg/l BAP, where the highest number of shoots per explant was 5.03±0.69. The directly induced shoots were multiplied and elongated on MS medium containing 1.0 mg/l BAP and 0.5 mg/l IBA, the number and length of regenerated shoots per culture being 6.07±0.39 and 5.84±0.65 cm, respectively. The best response to root induction (86.67%) was observed when shoots were transferred to ½ strength of gelled MS medium supplemented with 1.5 mg/l IBA within 16-24 days, with 13.60±1.76 roots per shoot unit. The well-rooted shoots were successfully acclimated in a mixture of soil, sand, and compost (1:1:1) with a survival rate of 88%. Jahangirnagar University J. Biol. Sci. 11(1 & 2): 31-40, 2022 (June & December)
Micropropagation of Dracaena fragrans cv.Victoria was conducted using the young, tender and disease-free leaves and nodal segments as explants collected from the local market of Savar, Dhaka. Surface sterilization of the explants pretreated with a liquid detergent and then 0.2% HgCl2 for 4-5 minutes produces maximum contamination free explants without any toxicity. After surface sterilization, different explants were inoculated on gelrite gelled MS medium supplemented with different concentrations of 2,4-D for callus induction and with different concentrations and combinations of BAP and NAA for direct shoot induction. Nodal explants showed high callus induction potentiality (80%) on MS medium supplemented with 1.5 mg/l 2,4-D. The highest frequency of direct shoot induction from nodal segment was 80% and the number of shoots per nodal segment was(5.28±1.17) when they were cultured on MS medium supplemented with 3.0 mg/l BAP and 0.3 mg/l NAA. The highest shoot multiplication (83.33%) with maximum number of shoot per unit callus (5.62±1.24) and maximum shoot length (3.27±0.82 cm) was observed when the nodal calli were transferred in gelrite gelled MS medium in combination with 4.5 mg/l BAP and 0.5 mg/l NAA. Additionally, the incorporation of 4% sucrose and 10% coconut water with the above mentioned medium showed the satisfactory shoot growth and development with an average 7.84±1.30 shoots per unit of callus which was 4.21±0.78 cm in length. Moreover, addition of 3.0 mg/l GA3 with the above mention medium showed highest rate of shoot elongation (5.83±2.31cm). For root induction, in vitro raised shoots were transferred onto half-strength of MS liquid medium augmented with different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (75%) were observed in halfstrength MS liquid medium supplemented with 0.5 mg/l IBA. After appropriate rooting the plantlets were successfully acclimatized (85% survival) when they were cultured in polybag containing (1:1:1) garden soil, sand and compost mixture before transferred to soil. Regenerated plants were morphologically identical with mother plants and showed their uniform growth in field condition. Jahangirnagar University J. Biol. Sci. 8(2): 1-11, 2019 (December)
This study was undertaken to standardize an efficient protocol for in vitro propagation of an ornamental bamboo Bambusa tuldoides Munro using nodal segments as explants. For direct shoot induction, liquid MS medium containing 2.0 mg/l BAP and 1.0 mg/l TDZ gave a maximum response (84%) with maximum number of shoots (4.80 ± 0.49) per explant. These shoots were proliferated efficiently on agitated liquid MS medium with 3.0 mg/l BAP and 1.5 mg/1 TDZ, which resulted in the formation of 15.40 ± 1.21 shoots per culture with 6.96 ± 0.65 cm shoot length. Shoots gradually increased in number and length during the first four subculture cycles in the same media combination but declined in 5th sub-culture cycle. After the 4th sub-culture cycle, the number of shoots per culture was 20.40 ± 1.44 and shoot length was 7.92 ± 0.78 cm. The addition of 10% coconut water with the above mentioned medium resulted in satisfactory shoot growth and development with 24.20 ± 1.16 shoots per culture. Maximum (84%) rooting with the highest number of roots per culture (7.20 ± 0.37) was obtained from half-strength MS medium fortified with 3.0 mg/l IBA. Well rooted plantlets were successfully acclimatized to the soil where the survival rate was 92%. Plant Tissue Cult. & Biotech. 32(2): 157-166, 2022 (December)
An experiment was conducted to develop a suitable in vitro plant regeneration technique for Rosa hybrida L. cv. ‘Double Delight’ using nodal segments and leaf tissues as explants through direct and indirect organogenesis. Aseptic explants were inoculated onto gelled MS medium contained various strengths of plant growth regulators alone and in combinations for callus and direct shoot induction. MS medium containing 2.0 mg/l BAP was found to be the most effective for direct shoot induction from nodal explants (87.5%) as it produced the maximum number of shoots per explant. The highest callus induction frequency was found to be 80% in leaf tissue, 72% in nodal segments and 96% in leaf and intermodal segments of in vitro raised plantlets in MS medium containing 2.5 mg/l 2, 4-D. The best percentage of indirect shoot organogenesis (93.33%) with maximum number and length of shoot were found when the different explant-derived callus was transferred in 3.0 mg/l BAP supplemented medium. The direct and indirectly induced shoots were multiplied on MS medium containing 3.0 mg/l BAP, where the average number and length of shoots per culture were 7.20 ± 0.80 and 5.22 ± 0.47 cm, respectively. Maximum rooting (80%) was observed in ½-strength of gelled MS medium containing 15.0 g/l sucrose with 1.0 mg/l IBA. Plantlets with the proper root system were then placed in a polybag with a 1:1:1 ratio of sand, garden soil, and compost, and they had a survival rate of about 76%. Plant Tissue Cult. & Biotech. 33(1): 25-36, 2023 (June)
The present experiment was conducted to establish an efficient protocol for mass propagation of Dendrocalamus giganteus Wall. ex Munro through in vitro culture using nodal segments as explants. The highest percentage of shoot induction (86.67 %) was obtained in the liquid MS medium supplemented with 1.5 mg/l BAP and 1.0 mg/l TDZ with maximum number of shoots (3.91 ± 0.87) per explant. The highest number and length of shoots were 22.83 ± 1.30 shoots/culture and 7.71 ± 0.96 cm, respectively induced on agitated liquid MS medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 8 % coconut water in the above mentioned medium resulted satisfactory shoot growth and development. The best response towards root induction (90 %) was observed when 3-4 shoots clump were transferred onto halfstrength of gelled MS medium fortified with 4.0 mg/l IBA with 9.31 ± 1.45 roots per unit shoot. Successful acclimatization of well-rooted clumps of 4-5 shoots was achieved in a mixture of soil, sand, and compost (1 : 1 : 1) with 90% survival rate. Bangladesh J. Bot. 52(2): 337-343, 2023 (June)
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