In this work, copper substituted cobalt ferrite nanoparticles Co1-xCuxFe2O4 (×=0, 0.3, and 0.7), has been synthesized via laser assisted hydrothermal synthesis (LAHS) methods. The XRD patterns showed single phase spinel structure, comparative study between the two preparation methods, effect of laser irradiation on the structural properties, and average crystallite size that is evaluated from most intense peak (311) utilizing Scherrer formula. Further studies for laser assisted hydrothermal as-prepared ferrite samples using each of Field emission-scanning electron microscope (FE-SEM) which revealed the study of topography, shape, particle size, vibrating sample magnetometer (VSM) with in 15kOe as maximum field. That was used to study the magnetic properties and confirmed the super-paramagnetic property of the prepared ferrite nanoparticles. Coercivity (Hc), remanence (Mr), saturation magnetization (Ms) and sequarness ratio were directly extracted from hysteresis loops. Moreover, results showed that the magnetic characteristics are on the basis of each particle size as well as cation distribution. In addition, the antibacterial property of the prepared nanoparticles against S. aureus and E. coli found to be improved after substitution of Cu in Co ferrite matrix.
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