Depletion of fossil fuel increased direction towards renewalable energy sources. Microbial Fuel Cell substitute technology for converting waste water from industry effluent as well meet the requirement for environmental problem. Study was made to treat the distillery effluent as substrate in mfc for bio-energy considering effect of oxygen flow rate, pH and substrate concentration. Different organic load were used in mfc for power generation and different oxygen flowrate for oxidation of proton coming from anode chamber effect of pH also keep consideration regading microbial growth of saccharomyces as a biocatalyst. The maximum power generation were observed at optimized condition of oxygen flow rate 250rpm, pH6 and substrate 60% (in form of organic load) at 0.9114 mW and current density 82.48 mA/m 2 .
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