. Molecular typing of canine parvovirus from Sulaimani, Iraq and phylogenetic analysis using partial VP2 gene. Bulg. J. Vet. Med., 20, No 3,[225][226][227][228][229][230][231][232][233][234][235] Canine parvovirus (CPV) remains the most significant viral cause of haemorrhagic enteritis and bloody diarrhoea in puppies over the age of 12 weeks. The objective of the present study was to detect and genotype CPV-2 by polymerase chain reaction (PCR) and to perform phylogenetic analysis using partial VP2 gene sequences. We analysed eight faecal samples of unvaccinated dogs with signs of vomiting and bloody diarrhoea during the period from December 2013 to May 2014 in different locations in Sulaimani, Kurdistan, Iraq. After PCR detection, we found that all viral sequences in our study were CPV-2b variants, which differed genetically by 0.8% to 3.6% from five commercially available vaccines. Alignment between eight nucleotides of field virus sequences showed 95% to 99.5% similarity. The phylogenetic analysis for the 8 field sequences formed two distinct clusters with two sequences belonging to strains from China and Thailand and the other six -with a strain from Egypt. Molecular characterisation and CPV typing are crucial in epidemiological studies for future prevention and control of the disease.
Molecular characterization of the chicken anemia virus by using the VP1 gene of CAV/ SK/2017 strains, Iraq ABSTRACT Chicken infectious anemia virus (CIAV) is one amongst the numerous pathogens in the poultry industry. CIAV infection can cause restraint of the immune system, the excitement of co-infections, vaccination failures and mortality. chicks. Maternal antibodies not prevent infection but to prevent these symptoms, immunological disorder, or transmission of the virus. Genetic characterizations for five sequences of chicken infectious anemia virus (CIAV) from different flocks of broiler industrial chickens were a consideration for the primary time in Iraq. Phylogenetic analysis of the viral protein 1 (VP1) gene, as well as the hypervariable region of the CIAV genome, indicated that Iraqi CIAV strains have belonged into genotype II. Amino acid comparison exhibit that the diversity of VP1 is indicated that the new strains were extremely pathogenic viruses. Our epidemiological study provided new insights into the prevalence of CIAV strain in recent years in Sulaimani province/Iraq.
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