This work is carried out with Gardenia coronaria leaves that belong to the family Rubiaceae, which is a small-to-medium-sized but tall, deciduous tree, 7.6–9 m high on an average. Leaves are used for the treatment of rheumatic pain and bronchitis. The leaf of the plant consists of coronalolide, coronalolic acid, coronalolide methyl ester, ethyl coronalolate acetate triterpenes (secocycloartanes), and so forth. Methanol extract from the leaves of Gardenia coronaria was completely screened for membrane stability and antibacterial activity. The lower concentrations of Methanolic leaf extract of Gardenia coronaria gave good antimicrobial and anti-inflammatory activity, but higher concentrations gave relatively more projecting antibacterial activity in vitro as compared with Kanamycin. The crude drug's anti-inflammatory effects were compared with those of Aspirin as positive control. The Methanolic extracts of Gardenia coronaria leaves possessed a broad spectrum antibacterial activity against a variety of both Gram-negative and Gram-positive organisms like Streptococcus agalactiae, Escherichia coli, Pseudomonas aeruginosa, Bacillus cereus, Shigella sonnei, Shigella boydii, and Proteus mirabilis, with a zone of inhibition from 10 to 16 mm. The extract also showed good membrane stability to be considered as having significant anti-inflammatory action.

The aim of our study was to find out the preliminary cytotoxic and thrombolytic effect of the seven selected medicinal plant leaves extract. In cytotoxic activity, out of the seven leaves extract three of them exhibited stronger brine shrimp lethality with LC 50 122.548 (Uncaria acida), 170.861 (Leea indica) and 175.469 (Piper porphyrophyllum) μg /mL respectively, and on MCF-7 cell line, they also exhibited moderate cytotoxic activity with different concentration of the extract of the same plant leaves such as,Uncaria acida (72.31, 56.22, 38.12 and 9.24%), Leea indica (67.31, 58.22, 43.12 and 15.24%), Piper porphyrophyllum (65.88, 48.12, 40.12 and 21.34%). In thrombolytic assessments, all the leaves extract showed moderate (considering > 20% moderate; **p < 0.01; *p < 0.05) clot lysis activity, but among the extracts, Uncaria cordata (27.36 ± 0.10%) showed the highest and Stachytarpheta indica the lowest (6.14 ± 0.20%) percent clot lysis as compared with the standard streptokinase (65.15 ± 0.16%). This study was conducted to legalize the folkloric use of seven medicinal plant leaves.

The apicomplexan parasite Cryptosporidium is well-known for its capability to induce cryptosporidiosis, a severe diarrheal disease in human and animals. Cryptosporidium can also be a potential pathogen in human for cancer progression, particularly colorectal cancer. This review was designed to outline the information about the life cycle of the Cryptosporidium, the consequences of Cryptosporidium infection into the response mechanism in immune compromised host and finally the regulation of oncomiRNAs and tumor suppressor miRNAs upon Cryptosporidium infection. Host-Cryptosporidium interaction caused alteration of expression of a series of microRNAs or miRNAs as a result of controlling defense mechanism. Regulation of miRNAs in the infected cells may be identified as possible biomarkers in cancer progression. Upregulation of oncomicroRNAs or oncomiRNAs and the downregulation of tumor suppressor miRNAs in the host epithelial cells due to the Cryptosporidium infection may lead to cancer initiation on human.

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Aim: From time past till date, proper documentation of the use of medicinal plants are helpful in drug development and research. The objective of this study is to evaluate several plant extracts of Bangladesh for their thrombolytic activity. Study Design: There are seven different plants from unlike families were studied in this primary research work. They were collected between August-September ( 2012) and the thrombolytic effect of their extractions was investigated. Streptokinase was used as standard to compare with and evaluate the significance of each result. Result: Among all studied plants Gardenia coronaria showed most promising result of 49.61±0.866% of lysis, whereas streptokinase exhibited a lysis of 75.36±0.964%. The extracts of Hedychium thyrsiforme and Artocarpus chaplasha showed also promising activity with 48.39±1.813% and 43.69±0.906% of thrombolytic effect, respectively. Conclusion: All the plants used in this study showed promising thrombolytic activity compared to standard. Proper phytochemical characterization and isolation of the constituent compounds responsible for this activity may be further investigated and could be a future source of lead thrombolytic compounds.