Backgrounds: Bacteriophage therapy could be an alternative strategy for the treatment of antibiotic-resistant bacteria. This study aimed to evaluate the antibacterial activities of isolated bacteriophages against methicillin-resistant Staphylococcus aureus (MRSA) isolates. Materials & Methods: A total of 16 clinical isolates of MRSA were collected from medical diagnostic laboratories in Tehran, Iran. A specific bacteriophage was isolated from hospital sewage using double-layer agar. Phage morphology was evaluated by transmission electron microscopy (TEM). Different bacteria were selected to determine the bacteriophage host range using spot test. Phage susceptibility to temperature and pH was evaluated by doublelayer agar method. In vitro assay was carried out on human epithelial type 2 (HEp-2) cells to investigate the effect of bacteriophage on the adhesion of MRSA to human epithelial cells. Findings: TEM suggested the Myoviridae family for the isolated phage. The effective titer of bacteriophages was 1.8脳10 7 PFU/mL. The isolated bacteriophage was stable at 4 藲C and pH=8. The isolated bacteriophage was specific for all clinical isolates of MRSA and had no lytic activity against other pathogenic bacteria. In evaluating the binding and invasion of MRSA to the HEp-2 cell line, as expected, the lytic activity of specific bacteriophages was observed following inoculation. Conclusion:The specificity and lytic activity of this phage on MRSA and MRSA-infected HEp-2 cell line emphasized that the isolated bacteriophage may serve as an effective prophylactic and alternative therapeutic agent in hospital settings.
Determining the mental model of experts regarding the factors hindering the development of table tennis in Iran. The purpose of this study is to determine the mental pattern of experts regarding the barriers to professional table tennis in Iran using Q methodology. The present study is applied in terms of purpose; In terms of exploratory nature; In terms of data collection, descriptive-survey, and due to application, Q methodology is a type of mixed research (quantitative-qualitative). The statistical population was veterans and elites in table tennis in Iran, from which 21 people were selected as participants through a combination of purposeful and unlikely sampling and snowball sampling. Participant sorting data were entered in SPSS software to identify the different mindsets of the participants using Q-factor analysis. Finally, the main deterrents in Iranian professional table tennis were prioritized based on nine mental patterns. These mental patterns were then discussed and interpreted. Finally, coping strategies were proposed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations鈥揷itations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright 漏 2024 scite LLC. All rights reserved.
Made with 馃挋 for researchers
Part of the Research Solutions Family.