Entomopathogenic fungus-Aschersonia sp.was found in the potted plants in the experimental sites of the Department of Zoology, Jahangirnagar University, Bangladesh. Using tissue planting technique, fungus colony was recovered on PDA. Morphology characterization based on mycelium, conidia, colony features as well as sequencing of rDNA of isolated fungus resulted as Aschersonia sp. Vegetative growth of the fungus on different fungal culture media, temperature, pH, and the light were conducted. The optimum temperature, pH and light regimes for mycelial growth were recorded at 20°C, pH 7, alternate light and dark condition. The suitable mycelial growth of the fungus was obtained on potato sucrose agar, Richard agar, carrot agar media over ten different culture media studied. To the best of our knowledge, the occurrence of Aschersonia sp. in Bangladesh is a new record.