Tea (Camellia sinensis L.) is recalcitrant to Agrobacterium-mediated genetic transformation largely due to the bactericidal effects of tea polyphenols and phenolics oxidation induced by necrosis of explant tissue over the process of transformation. In this study, different antioxidants/adsorbents were added as supplements to the co-cultivation and post co-cultivation media to overcome these problems for the transformation improvement. Tea-cotyledon-derived calli were used as explants and Agrobacterium rhizognes strain ATCC 15834 was used as a mediator. Results showed that Agrobacterium growth, virulence (vir) gene expression and browning of explant tissue were greatly influenced by different supplements. Murashige and Skoog (MS) basal salts medium supplemented with 30 g·L−1 sucrose, 0.1 g·L−1
l-glutamine and 5 g·L−1 polyvinylpolypyrrolidone (PVPP) as co-cultivation and post co-cultivation media could maintain these parameters better that ultimately led to significant improvement of hairy root generation efficiency compared to that in the control (MS + 30 g·L−1 sucrose). Additionally, the reporter genes β-glucuronidase (gusA) and cyan fluorescent protein (cfp) were also stably expressed in the transgenic hairy roots. Our study would be helpful in establishing a feasible approach for tea biological studies and genetic improvement of tea varieties.
This paper presents data related to an article entitled “Green tea flavor determinants and their changes over manufacturing processes” (Han et al., 2016) [1]. Green tea samples were prepared with steaming and pan firing treatments from the tender leaves of tea cultivars ‘Bai-Sang Cha’ (‘BAS’) and ‘Fuding-Dabai Cha’ (‘FUD’). Aroma compounds from the tea infusions were detected and quantified using HS-SPME coupled with GC/MS. Sensory evaluation was also made for characteristic tea flavor. The data shows the abundances of the detected aroma compounds, their threshold values and odor characteristics in the two differently processed tea samples as well as two different cultivars.
Tea volatiles’ generation and retention over manufacturing processes are crucial for tea quality. In this study, floral volatile adsorption and retention in green tea scented with Jasminum sambac flowers were examined over the scenting process. Out of 34 enhanced volatiles in the scented tea, β-ionone, β-linalool, indole, and methyl anthranilate were the most potent odorants with 5.1–45.2-fold higher odor activity values than the corresponding controls in the nonscented tea. Scenting efficiencies for the floral volatiles retained in the scented tea (the percentage of volatile abundance over its corresponding amount in jasmine flowers) ranged from 0.22% for α-farnesene to 75.5% for β-myrcene. Moreover, due to additional rounds of heat treatment for scented green tea manufacturing, some volatiles such as carotenoid-derived geraniol and β-ionone and lipid-derived (Z)-jasmone were heat-enhanced and others such as nonanal were heat-desorbed in the scented green tea. Our study revealed that dynamic volatile absorption and desorption collectively determined tea volatile retention and tea aroma. Our findings may have a great potential for practical improvement of tea aroma.
The study was conducted to investigate the soil quality including heavy metal concentrations in agricultural lands around dyeing, glass and textile industries at Tangail district of Bangladesh. A total of nine samples, three samples from each industrial site, were collected at a depth of 0-15 cm with an interval of 10 m from each point of the agricultural lands adjacent to selected industries for analyzing the soil chemical properties such as pH, OM, total N, available P and S including heavy metals (Pb, Cd, Ni, Cu and Cr) concentrations. The study found soil pH of 6.4 and 6.1 around textile and glass industry, respectively, while comparatively lower pH (4.4) was observed around dyeing industry. Comparatively higher levels of OM, total N, available P and S were found in soil around dyeing industry, whereas lower levels of OM and available S were observed around textile industry. The Cu, Pb and Cr were the dominant heavy metal around dyeing, glass and textile industry, respectivelyJ. Environ. Sci. & Natural Resources, 10(2): 109-116 2017
The main purpose of the study was to explore the problem confrontation of vegetable growers in production and marketing of vegetables in the selected areas of Bogra district of Bangladesh. The study also aims to explore the relationships between the selected socio-demographic characteristics of the respondents and their problem confrontation in case of production and marketing of commonly cultivated vegetables. Data were gathered from 100 randomly sampled vegetable growers of Atmul and Mokamtola union under Shibganj upazila (Sub-district) during the period of 25 January to 20 February, 2020 by combining a mix of different methods such as survey, group discussion and Key Informant Interview (KII) method. Ten selected socio-demographic characteristics of vegetable growers namely age, education, household size, farm size, annual family income, farming experience, organizational participation, training experience, access to credit and extension media contact were considered to investigate the relationships with the problem confrontation of vegetable growers in case of production and marketing of most commonly cultivated vegetables. Most of the vegetable growers (77%) had faced medium level of problems compared to 19% of the respondents had faced high level of problems while only 4% of the respondents had faced low level of problems in case of production and marketing of vegetables. Correlation analyses indicated that socio-demographic attributes of the respondents namely household size, farm size, annual family income, access to credit and training experience had significant contributions towards problem confrontation of vegetable growers in production and marketing of vegetables. It is evident that vegetable growers faced range of problems in case of production and marketing of commonly grown vegetables. This research gears towards realization of problem confrontation scenario of vegetable growers and to take proper initiatives by the policy makers of agriculture sector to improve the productivity of vegetables in Bangladesh.
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