A precise, sensitive eco-friendly, simple, rapid, and derivative spectrofluorimetric method was developed to quantify edoxaban tosylate monohydrate in pure form and pharmaceutical dosage form. Sudden death due to pulmonary embolism as a consequence of coronavirus infection (covid-19) is an emerging problem. As a result, the world health organization introduced new guidelines to treat patients with COVID-19 with oral anticoagulants. Edoxaban tosylate monohydrate is an oral anticoagulant that doesn’t require hospitalization after dose adjustment. This spectrofluorimetric method relies on the derivatization by 9-fluorenyl methyl chloroformate at room temperature in borate buffer pH 9.0. After excitation at 265 nm, the product is highly fluorescent at 309 nm. Many experimental factors influencing the reaction's stability and development were thoroughly investigated and optimized. The method validation was evaluated by using ICH guidelines and showed high precision and accuracy with an average percent recovery of 101.46% ± 1.02. The linear range was 5.0–50.0 ng/mL with a correlation coefficient of 0.9999, the LOD was 1.5 ng/mL, and the LOQ was 4.5 ng/mL. The green assessment of the method was achieved utilizing the eco-scale and the Green Analytical Procedure Index. There was no significant difference between the results of the suggested method and those of the reported method according to Statistical analysis.
Thromboembolism because of coronavirus infection (COVID-19) resulted in increasing the number of sudden deaths after recovery. So, patients were switched to edoxaban tosylate monohydrate (EDTM) therapy after low molecular weight heparin to shorten the hospitalization period. In response to increase the demand for EDTM, we developed a home-made screen-printed electrode to determine EDTM in its pure form and pharmaceutical dosage form. Optimized conditions were achieved with a screen-printed electrode utilizing EDTM-Phosphotungstic acid (EDTM-PTA) as an ion pair complex and a plasticizer dibutyl phthalate with a Nernstian slope of 58.78 ± 0.1 mV decade−1 at the concentrations ranging from 5.0 × 10–6 to 1.0 × 10–2 M EDTM (R2 = 0.9985). The detection limit was 4.3 × 10–6 M. Our method was proven to be eco-friendly after assessment by Eco-Scale and Complex-GAPI. The results obtained were validated according to ICH guidelines and were found to agree with those gained from the reference method.
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