Problem statement: Extended endplate connection is one of the most widely used beam-to column steel connections because of its fabrication simplicity, good overall performance and cost effectiveness compared with other connection types. The objective of this research is to develop three-dimensional finite element models to study the behavior of large capacity eight-bolt extended un-stiffened wide endplate steel connections, using current-technology elements instead of legacy elements which were previously used by other researchers. Approach: A finite element software package (ANSYS, version 11.0) was used to create and analyze three finite element models. Two of the finite element models were compared with previously reported experimental results to validate the accuracy of the finite element models. The third model was based on a modification of the second finite element model to improve bolt force distribution. Eight-node brick solid elements were used to model the connection members. The bolt shank was modeled using one three-dimensional spar element that connected the bolt head and nut together. Pretension in bolts, contact algorithm and material nonlinearity were considered in the finite element models. Results: Results of the first and the second finite element models were compared with experimental data. The comparison was based on moment-beam rotation and moment-endplate separation of the finite element models and the corresponding tested specimens. The results of the finite element models were used to compare the behavior of the bolts in the tension region adjacent to the beam bottom flange. Conclusion: The comparison showed good correlations between the finite element models and the corresponding tested specimens which confirmed the validity of the proposed models. Thus, a modified connection was proposed to improve the connection response. A finite element model of the modified connection was modeled, analyzed and compared to the original finite element model prior to modification to show their correlation
G protein coupled receptor kinase 5 (GRK5) is localized within the nucleus to moderate functions such as DNA transcription, in addition to its localization at the plasma membrane. In this report, we show that GRK5 modifies the nucleolar stress response activated by the DNA polymerase inhibitor, Actinomycin D (ActD). We showed an increased sensitivity to the apoptotic effects of ActD on cervical HeLa and the breast cancer cell line MDA MB 231 with reduced GRK5 protein expressions. Furthermore, the rate of response to ActD varied between the two types of breast cancer cells tested, MDA MB 231 and MCF7 cells, since they have innate differences in GRK5 protein expressions. During the early ActD treatment period, GRK5 phosphorylates nucleophosmin (NPM1) at T199 before and during treatment. Phosphorylation at this site increases the ability of NPM1 to interact with p14ARFin vitro, which may affect p14ARF protein expression levels. Expression levels are lower in control shRNA transfected cells but higher in GRK5 shRNA transfected cells. Collectively, this suggests that GRK5 modifies the nucleolar stress response associated with ActD.
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