This study documented the reliability of oral swabbing when investigating yeast carriage in healthy denture wearers. Moreover, just a diagnostic tool, sampling upper dentures for Candida could be the opportunity to verify the patient's compliance to hygiene advice.
The present study demonstrates the in vitro and in vivo adsorption of peroxidase onto titanium surfaces. Titanium foils (mean ± SEM: 365 ± 2 mm 2 , n = 114) were incubated during 30 min with lactoperoxidase (4 mg in 5 mL 100 mM phosphate buffer pH 7). After 15 washings by H 2 O, titanium foils were incubated with o-phenylenediamine (6 mg/mL) and H 2 O 2 (7 mM) during 30 min. The reaction was then stopped by the addition of HCI 1M and the absorbance of the liquid phase was read on a spectrophotometer at 492 nm. In vitro adsorbed lactoperoxidase onto titanium surfaces was 0.70 ± 0.05 ng/mm 2 (mean ± SEM, n = 30). X-ray photoelectron spectroscopy confirmed the incorporation of pro-tein nitrogen onto titanium surfaces: the nitrogen atomic percentage increased from 0.9 ± 0.3 to 12.7 ± 0.2% (n = 3) and from 3.7 ± 0.1 to 14.4 ± 0.4% (n = 5) when titanium foils were incubated in the lactoperoxidase solution during 30 min and 24 h respectively. In vivo, oral peroxidases adsorbed on titanium healing abutments from 0.01 to 0.58 ng/mm 2 (n = 19) after 2 weeks in the oral environment.
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