BackgroundPesticide exposure possesses risk of genotoxicity to humans, particularly farmers. Despite accumulating evidences linking genotoxicity to pesticide exposure, epidemiological studies to address pesticide toxicity in occupationally exposed farmers in Malaysia remain underreported. Thus, this study was aimed to determine the presence of nuclear abnormalities through the assessment of micronucleus (MN) and binucleus (BNu) frequencies in exfoliated buccal epithelial cells from farmers who were exposed to pesticides. A cross-sectional study of farmers among different agricultural activities farmers in Bachok and Pasir Puteh, Kelantan, North East of Peninsular Malaysia was done to evaluate the presence of nuclear abnormalities and its correlation with their health status and farming activities.ResultsAnalysis of buccal cells revealed that the frequency of MN was significantly higher (p < 0.05) in farmers as compared to controls. In contrast, no significant difference (p > 0.05) was observed for BNu frequency in between groups. Correlation analysis showed that apart from a significant (p < 0.05) and positive correlation between the duration of fertilizers exposure and frequencies of MN (r = 0.42, P = 0.001) and BNu (r = 0.37, P = 0.02), no other correlation of various confounding factors on the formation of MN and BNu were observed.ConclusionIn conclusion, pesticide and fertilizers exposure may contribute to the promotion of nuclear anomalies among Malaysian farmers who are engaged in mixed plantation activities. Further assessment of larger populations is important to address and overcome the potential risk of pesticide-induced genotoxicity.
Pesticide exposure may cause genotoxic effects by inducing the formation of micronucleus (Mn). Mn are fragments of chromosomes that remains after cells division. The increase in Mn may increase the risk of cancer formation. Our study aimed to determine the effects of lifestyle and pesticide exposure on the formation of Mn in epithelial cells from buccal swabs among paddy farmers in Malaysia. About 40 farmers who were exposed to pesticides were chosen as subjects and 30 personnels whose not directly exposed to pesticides, were chosen as the control group. Demographic and anthropometric data were obtained from questionnaires developed. Analysis of Mn formation was done using Giemsa staining (10% v/v) and the frequency of Mn formation was scored from 1000 cells per sample. Kruskal-Wallis test done between Mn frequency with age group showed a significant increase (p < 0.05) in Mn frequency in farmers as compared to the control in the age group of 30-39 , 40-49 years, and 50-59 years. Significant increased (p < 0.05) were observed between Mn frequency groups of normal BMI, pre-obese, and grade 1 obese as compared to control. Significant increase of Mn frequency (p < 0.01) was also seen among smokers and farmer's group (15.39 ± 3.34) as compared to controls (4.76 ± 1.26). The maximum numbers of Mn found in farmers are 7 Mn per cell whereas for control group is only 3 Mn. However, most farmers had only 1 Mn (81.75 ± 6.42%) and 2 Mn (15.28 ± 5.14%). Mn frequency with the duration of exposure to pesticides in a month and the use of PPE revealed no significant difference (p = 0.27). In conclusion, the increased frequency of Mn was influenced by age, gender, BMI and smoking status of farmers besides commonly repeated duration of exposures and the use of PPE. Further studies are needed to analyze the causes of an increased in Mn among farmers.
Health awareness promotion among farming communities are important for a sustainable agriculture activities. A crosssectional study was conducted to assess health status among farming communities in Cameron Highlands, Pahang (n = 61) and Bachok and Pasir Puteh, Kelantan (n = 143
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.