Expression of uPAR (CD87) may play a relevant role in the diagnosis and pathophysiology and prognostic pattern of acute myeloid leukemia. The aims of this study were to find out the prognostic significance of pretreatment detection of CD87 and study the prevalence of CD87 expression and its value as a predictor for survival in AML patients. CD87 expression has been studied on blasts in 110 newly diagnosed AML patients. Immunophenotyping and cytogenetic analysis of these cases were performed. CD87 was positive in 80 (72.7%) cases of patients. The MFI categorized the expression of CD87 into CD87(High) and CD87(Low) expression. Blast cells show that monocytic differentiation has a significantly more CD87(High) expression than CD87(Low) expression. Cytogenetic abnormalities were found in 58.7% of patients with CD87(Low) AML and 41.25% of patients with CD87(High) AML. Cases with CD87(High) expression cells were characterized by a significantly lower survival period especially when co-expressed with CD56, CD34, and/or CD64. There is a negative prognostic influence of the expression of CD87 on the surface of AML blasts, but more tests are necessary to explain the pathophysiological mechanisms behind these findings and to learn about the mechanism that influences the CD87 expression and function.

Mastitis is a multifactorial and ubiquitous disease that results from interactions between the host, environment, and infectious agents leading to extensive economic losses. The objectives of this study were to estimate the prevalence of S. aureus in clinical and subclinical mastitis in Holstein dairy cows and determine the susceptibility of S. aureus isolatesagainst different antibiotics for screening of antibiotic resistance genes. A total of 415 Holstein dairy cows were randomly selected from three Egyptian governorates. Selected cows were examined for clinical and subclinical mastitis during the period from October 2014 to June 2018. Milk samples were examined for the presence of S. aureus. The S. aureus isolates were subjected to antibiotic sensitivity, molecular identification, and detection of the antibiotic resistance gene.The prevalence of S. aureus isolated from milk samples was 15.4% of which, 14.3% in clinical cases, and 15.7% in subclinical cases.The antibiogram of S. aureus isolates against 13 antibiotics using the disc-diffusion method revealed the highest rate of resistance to Oxacillin (OX) (96.7%), followed by Ampicillin (AM),Cefoxitin (FOX) (93.3%, each), Tetracycline (TE) (73.3%), Cefotaxime (CTX) (70%), Ampicillin/Sulbactam (SAM) (66.7%), Erythromycin (E) and Sulphamethoxazole/Trimethoprim (SXT) (56.7%, each), Gentamicin (GM) (53.3%), Ofloxacin (OFX) (40%), Chloramphenicol (C) (36.7%), Ciprofloxacin (CIP) (30%) and finally Vancomycin (VA) (0%). Molecular PCR assay revealed that all the 16 S. aureus isolates (100%) carried mecA gene, while 15 out of 16 isolates (93.7%) carried blaZ genebut, 8 out of 16 (50%) carried tetK gene, and only one isolate (0.06%) carried fexA gene. Uncontrolled uses of antibiotics in the treatment of mastitis should be restricted and increase awareness about the risk of antimicrobial-resistant bacteria in milk.

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