The study investigated the relationship between the overall scientific performance of the pre-test and post-test and the impact of the application of "Microb" on scientific performance. The theme is "microorganisms and their effects". The sample group consisted of 30. Year 5 pupils’ from a primary school in Johor Bahru. They were selected for this quantitative study through purposeful sampling. Investigation, pre-test and post-test are used to collect research data. The results show that based on the four science constructs, year 5 pupils’ acceptance of the “Microb” mobile application is very high. According to the survey results, although there is no relationship between the two overall performance tests and no impact was found in the research, it was found that the “Microb” mobile application has a positive impact on the scientific performance of year 5 pupils’, based on the construct of scientific process skills, understanding, application and knowledge. The results of this research will make an important contribution to the field of education and provide new options for choosing the "Microb" mobile application as an educational tool to strengthen science education. In general, the "Microb" mobile application in technology can be a good educational tool for scientific learning, but educators must organize lesson plans well and not deviate from the focus of learning
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