Effect of three medium types, Murashige and Skoog (MS), Linsmaier and Skoog (LS) and Lloyd and McCown (WPM), two explant types (shoot-tip and single node cutting), three cytokinin types (Benzyladenine, Kinetin and 2-isopenteny1 adenine), two additives; viz., s (thiamine and pyridoxine hydrochloride) and amino acids (asparagine and arginine) and two gelling agents (guar seed powder and locust bean seed powder) combined with agar on micropropagation of Haplophyllum tuberculatum were investigated. For rooting, the effect of three auxins (Indole acetic acid, Indol butyric acid and Naphthalene acetic acid) were studied in combination with three medium strengths (full, half and quarter) combined with the best mixture (0.50 mgl-1 Kin + 6 gl-1 agar with 2 gl-1 guar seed powder) of gelling agents. Obtained results showed that Murashige and Skoog (MS) was the best medium with single node explant as starting material during establishment stage. Multiple shoots were obtained on MS medium supplemented with 1.0 mgl-1 Kinetin (Kin) combined with 0.1 mgl-1 α-naphthalene acetic acid (NAA). Thiamine and arginine were the best vitamins and amino acid at 0.1 mgl-1 and 0.50 mgl-1 , respectively. Agar at 6 gl-1 + guar seed powder at 2 gl-1 was the best gelling agent treatment. Half strength MS medium with 0.50 mgl-1 Kin + 0.5 mgl-1 NAA and 6 gml-1 agar with 2 gml-1 guar seed powder was the best combination for rooting were successfully (85% survival) acclimatized in mixture of peatmoss, vermiculite, washed sterilized sand and perlite (1:1:1:1, V/V/V/V).
This study was carried out during 2011/2012 and 2012/2013 seasons to throw some lights on the effect of nine chemical and natural rest breaking agents. (Dormex at 4%, brotacion at 4%, garlic oil at 5%, sodium azide at 1%, thiourea at 2%, oils of flax seed, clove and onion each at 5% and H 2 O 2 at 5%) on breaking dormancy and improving productivity of Superior grapevines.Using any one of the nine chemical and natural rest breaking agents was very effective in advancing the start and end of bud burst, shortening duration of bud burst and blooming periods and improving the percentages of bud burst and fruiting buds, all growth aspects, leaf pigments and nutrients, berry setting%, yield and quality of the berries relative to the check treatment. The best chemical and natural rest breaking agents were dormex at 4%, brotacion at 4% and garlic oil at 5%, respectively.Subjecting the vines to dormex or brotacion each at 4% gave the best results with regard to breaking dormancy and promoting the productivity of Superior grapevines. Under organic farming system, it is suggested to use garlic oil at 5%.
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