No abstract

Tissue-dwelling helminths are known to induce intestinal and systemic inflammation accompanied with host compensatory mechanisms to counter balance nutritional and metabolic deficiencies. The metabolic and immune responses of the host depend on parasite species and tissues affected by the parasite. This study investigated metabolic and immuno-inflammatory responses of mice infected with tissue-dwelling larvae of Trichinella zimbabwensis and explored the relationship between infection, metabolic parameters and Th1/Th17 immune responses. Sixty (60) female BALB/c mice aged between 6 to 8 weeks old were randomly assigned into T. zimbabwensis-infected and control groups. Levels of Th1 (interferon-γ) and Th17 (interleukin-17) cytokines, insulin and blood glucose were determined as well as measurements of body weight, food and water intake. Results showed that during the enteric phase of infection, insulin and IFN-γ levels were significantly higher in the Trichinella infected group accompanied with a reduction in the trends of food intake and weight loss compared with the control group. During systemic larval migration, trends in food and water intake were significantly altered and this was attributed to compensatory feeding resulting in weight gain, reduced insulin levels and increased IL-17 levels. Larval migration also induced a Th1/Th17 derived inflammatory response. It was concluded that T. zimbabwensis alters metabolic parameters by instigating host compensatory feeding. Furthermore, we showed for the first time that non-encapsulated T. zimbabwensis parasite plays a role in immunomodulating host Th1/Th17 type responses during chronic infection.

To improve diagnostic tools, immunotherapies and vaccine development for trichinellosis surveillance and control there is a need to understand the host immune responses induced during infection with Trichinella zimbabwensis, a tissue-dwelling nematode. In this study, we sought to determine immune responses induced in mice during T. zimbabwensis infection. The parasite strain used (Code ISS1209) was derived from a naturally infected crocodile (Crocodylus niloticus) and is the main Trichinella species prevalent in southern Africa. Sixty 6- to 8-week-old female BALB/c mice were randomly assigned to two equal groups: T. zimbabwensis-infected (n= 30) and the non-infected control group (n= 30). Levels of serum tumour necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), interleukin-4 (IL-4) as well as parasite-specific IgM, IgG, IgG1, IgG2a, IgG2b and IgG3 antibody responses were determined using enzyme-linked immunosorbent assay (ELISA). The cytokines and antibodies provided information on T-helper 1 (Th1)- and Th2-type, T-regulatory and antibody responses. Results showed that during the intestinal stage of infection, higher levels of parasite-specific IgM, IgG, IgG1 (P < 0.05) and IL-10 and TNF-α (P < 0.001) were observed in the Trichinella-infected group compared with the non-infected control group. In the parasite establishment and tissue migration phases, levels of IgG1 and IgG3 were elevated (P < 0.001), while those of IgM (P < 0.01) declined on days 21 and 35 post infection (pi) compared to the enteric phase. Our findings show that distinct differences in Th1- and Th2-type and T-regulatory responses are induced during the intestinal, tissue migration and larval establishment stages of T. zimbabwensis infection.

No abstract

Preliminary laboratory studies were conducted to determine the molluscicidal potency of Jatropha curcas, the physic nut. Biomphalaria glabrata and Bulinus globosus snails were exposed to varying concentrations of aqueous extracts of crushed J. curcas seeds from unripe, ripe and overripe fruits collected from two geographically different sites, (Bindura and Kariba, Zimbabwe). Snail mortalities were compared between different developmental stages of J. curcas and between seed collection areas, and LC 50 and LC 90 values for the different extracts tested were computed. Biomphalaria glabrata was most susceptible to unripe fruit seed extract (with LC 50 values of 282 and 389 mg l -1 being recorded for Kariba-origin and Bindura-origin plants, respectively) and least susceptible to ripe fruit seed extracts (with LC 50 values of 605 and 708 mg l -1 being recorded for Karibaorigin and Bindura-origin plants, respectively). Bulinus globosus was most susceptible to overripe fruit seed extract (Kariba-origin plants: LC 50 , 389 mg l -1 ) and least susceptible to unripe fruit seed extract (Kariba-origin plants: LC 50 , 687 mg l -1 ). The area from which fruits were collected did not influence the potency of J. curcas. The potency of J. curcas depends on both the developmental stage of the fruit and the species of the target snail. In view of its many other uses, besides as a molluscicide, we recommend further studies on J. curcas.