Biosolids are waste from wastewater treatment and have a high content of organic matter and nutrients. In this study, not conventional physicochemical and biological properties of biosolids produced during different seasons of the year were evaluated. These properties are not considered in environmental regulations; however, they are of agronomic interest as indicators of quality and functionality in soils. Also, molecular analysis by Fourier-transform infrared (FTIR) was conducted, enzymatic analysis using the APIZYM® system was performed and two indices of functional and microbial diversity were established. The results showed that the biosolids had a high content of total organic carbon, total nitrogen, P, and K. FTIR analyses showed that chemical composition of biosolids was similar during all year. The C and N of microbial biomass demonstrated presence of active microorganisms, as well as a uniformity in its richness and abundance of species that could present a positive synergy with soil microorganisms. The enzymatic activities showed that the biosolids contained an enzymatic machinery available to promote the mineralisation of the organic matter of biosolids and could even transcend into the soil. Finally, biological properties can be used as indicators of quality and functionality of biosolids before being used as an organic amendment, especially in agricultural soils.

The Bajío—Mexico’s central lowlands—is a region of economic importance because of its agricultural industry. Over time, agricultural practices have led to soil deterioration, loss of fertility, and abandonment. In this study, six agricultural soils were analyzed: AGQ, CTH, CTJ, JRM, CRC, and CYI, and used to develop a soil quality index (SQI) that includes the use of physicochemical, biological, and ecophysiological indicators to differentiate soil quality. Principal component analysis (PCA) was used, reducing the indicators from 46 to 4, which represents 80.4% of data variability. It was implemented the equation of additive weights using the variance of the principal components as a weight factor for the SQI. The developed SQI was according to the indicators WHC, SLT, N-NO3−, and qCO2, differentiating the quality of soils from the agricultural management in low quality (JRM < CYI < AGQ) and moderate quality (CTJ < CRC < CTH). The use of biological and ecophysiological indicators added to the PCA and the equation of additive weights allowed establishing an SQI with a minimum of indicators, sensitive to agricultural management, facilitating its interpretation and implementation for the Mexican Bajío region and soils in similar conditions around the world.

Adding biosolids to agricultural soils can improve its quality through increased storage of C and N. A study to analyze the short-term impact of the addition of biosolids on the release of nutrients and the microbial activity in agricultural soil was carried out. The microbial biomass C (MB-C), urease activity (UA), and mineralization of N and C at different application rates of biosolids were evaluated (0 mg, 100mg and 200 mg of N-NH4+ kg-1). In addition, a biosolid-only treatment was tested. It was observed an increase in C and N mineralization, NH3 volatilization, and MB-C content, according to the application rate of biosolids. In biosolid treatments, UA increased 100% on average in the first seven days of incubation. These results suggest that the nutrient content in the soil is improved and microbial activity is positively stimulated.