Leaf age-dependent changes in structure, nitrogen content, internal mesophyll diffusion conductance ( g m ), the capacity for photosynthetic electron transport ( J max ) and the maximum carboxylase activity of Rubisco ( V cmax ) were investigated in mature non-senescent leaves of Laurus nobilis L., Olea europea L. and Quercus ilex L. to test the hypothesis that the relative significance of biochemical and diffusion limitations of photosynthesis changes with leaf age. The leaf life-span was up to 3 years in L. nobilis and O. europea and 6 years in Q. ilex . Increases in leaf age resulted in enhanced leaf dry mass per unit area ( M A ), larger leaf dry to fresh mass ratio, and lower nitrogen contents per dry mass ( N M ) in all species, and lower nitrogen contents per area ( N A ) in L. nobilis and Q. ilex . Older leaves had lower g m , J max and V cmax . Due to the age-dependent increase in M A , mass-based g m , J max and V cmax declined more strongly (7-to 10-fold) with age than area-based (5-to 7-fold) characteristics. Diffusion conductance was positively associated with foliage photosynthetic potentials. However, this correlation was curvilinear, leading to lower ratio of chloroplastic to internal CO 2 concentration ( C c / C i ) and larger drawdown of CO 2 from leaf internal air space to chloroplasts ( D D D D C ) in older leaves with lower g m . Overall the agedependent decreases in photosynthetic potentials were associated with decreases in N M and in the fraction of N in photosynthetic proteins, whereas decreases in g m were associated with increases in M A and the fraction of cell walls. These age-dependent modifications altered the functional scaling of foliage photosynthetic potentials with M A , N M , and N A . The species primarily differed in the rate of agedependent modifications in foliage structural and functional characteristics, but also in the degree of agedependent changes in various variables. Stomatal openness was weakly associated with leaf age, but due to species differences in stomatal openness, the distribution of total diffusion limitation between stomata and mesophyll varied among species. These data collectively demonstrate that in Mediterranean evergreens, structural limitations of photosynthesis strongly interact with biochemical limitations. Age-dependent changes in g m and photosynthetic capacities do not occur in a co-ordinated manner in these species such that mesophyll diffusion constraints curb photosynthesis more in older than in younger leaves.
Soil respiration constitutes the second largest flux of carbon (C) between terrestrial ecosystems and the atmosphere. This study provides a synthesis of soil respiration (R s ) in 20 European grasslands across a climatic transect, including ten meadows, eight pastures and two unmanaged grasslands. Maximum rates of R s (R s max ), R s at a reference soil temperature (10°C; R s 10 ) and annual R s (estimated for 13 sites) ranged from 1.9 to 15.9 μmol CO 2 m −2 s −1 , 0.3 to 5.5 μmol CO 2 m −2 s −1 and 58 to 1988 g C m −2 y −1 , respectively. Values obtained for Central European mountain meadows are amongst the highest so far reported for any type of ecosystem. Across all sites R s max was closely related to R s 10 .Assimilate supply affected R s at timescales from daily (but not necessarily diurnal) to annual.Reductions of assimilate supply by removal of aboveground biomass through grazing and cutting resulted in a rapid and a significant decrease of R s . Temperature-independent seasonal fluctuations of R s of an intensively managed pasture were closely related to changes in leaf area index (LAI). Across sites R s 10 increased with mean annual soil temperature (MAT), LAI and gross primary productivity (GPP), indicating that assimilate supply overrides potential acclimation to prevailing temperatures. Also annual R s was closely related to LAI and GPP. Because the latter two parameters were coupled to MAT, temperature was a suitable surrogate for deriving estimates of annual R s across the grasslands studied. These findings contribute to our understanding of regional patterns of soil C fluxes and highlight the importance of assimilate supply for soil CO 2 emissions at various timescales.
The increases in diffusion limitations in older leaves and at higher light scaled with age-and light-dependent increases in M A and D F . Overall, our study demonstrates a large potential of foliage photosynthetic acclimation to changes in leaf light environment, but also highlights enhanced structural diffusion limitations in older leaves that result from leaf structural acclimation to previous rather than to current light environment and accumulation of structural compounds with leaf age.
The main determinants of soil respiration were investigated in 11 forest types distributed along an altitudinal and thermal gradient in the southern Italian Alps (altitudinal range 1520 m, range in mean annual temperature 7.8 1C). Soil respiration, soil carbon content and principal stand characteristics were measured with standardized methods. Soil CO 2 fluxes were measured at each site every 15-20 days with a closed dynamic system (LI-COR 6400) using soil collars from spring 2000 to spring 2002. At the same time, soil temperature at a depth of 10 cm and soil water content (m 3 m À3 ) were measured at each collar. Soil samples were collected to a depth of 30 cm and stones, root content and bulk density were determined in order to obtain reliable estimates of carbon content per unit area (kg C m À2 ). Soil respiration and temperature data were fitted with a simple logistic model separately for each site, so that base respiration rates and mean annual soil respiration were estimated. Then the same regression model was applied to all sites simultaneously, with each model parameter being expressed as a linear function of site variables. The general model explained about 86% of the intersite variability of soil respiration. In particular, soil mean annual temperature explained the most of the variance of the model (0.41), followed by soil temperature interquartlile range (0.24), soil carbon content (0.16) and soil water content (0.05).
Estimates of leaf gas-exchange characteristics using standard clamp-on leaf chambers are prone to errors because of diffusion leaks. While some consideration has been given to CO2 diffusion leaks, potential water vapour diffusion leaks through chamber gaskets have been neglected. We estimated diffusion leaks of two clamp-on Li-Cor LI-6400 (LiCor, Inc., Lincoln, NE, USA) leaf chambers with polymer foam gaskets and enclosing either 2 or 6 cm 2 leaf area, and conducted a sensitivity analysis of the diffusion leak effects on Farquhar et al. photosynthesis model parameters -the maximum carboxylase activity of ribulose 1·5-bisphosphate carboxylase/oxygenase (Rubisco) (Vcmax), capacity for photosynthetic electron transport (Jmax) and nonphotorespiratory respiration rate in light (Rd). In addition, net assimilation rate (An) versus intercellular CO2 (Ci) responses were measured in leaves of Mediterranean evergreen species Quercus ilex L. enclosing the whole leaf chamber in a polyvinyl fluoride bag flushed with the exhaust air of leaf chamber, thereby effectively reducing the CO2 and water vapour gradients between ambient air and leaf chamber. For the empty chambers, average diffusion leak for CO2, K CO2 , (molar flow rate corresponding to unit CO2 mole fraction difference) was ca. 0.40 mmol s -1 . K CO2 increased ca. 50% if a dead leaf was clamped between the leaf chamber. Average diffusion leak for H2O was ca. 5-to 10-fold larger than the diffusion leak for CO2. Sensitivity analyses demonstrated that the consequence of a CO2 diffusion leak was apparent enhancement of An at high CO2 mole fraction and reduction at lower CO2 mole fraction, and overall compression of Ci range. As the result of these modifications, Farquhar et al. model parameters were overestimated. The degree of overestimation increased in the order of Vcmax < Jmax < Rd, and was larger for smaller chambers and for leaves with lower photosynthetic capacity, leading to overestimation of all three parameters by 70-290% for 2 cm 2 , and by 10-60% for 6 cm 2 chamber. Significant diffusion corrections (5-36%) were even required for leaves with high photosynthetic capacity measured in largest chamber. Water vapour diffusion leaks further enhanced the overestimation of model parameters. For small chambers and low photosynthetic capacities, apparent Ci was simulated to decrease with increasing An because of simultaneous CO2 and H2O diffusion leaks. Measurements in low photosynthetic capacity Quercus ilex leaves enclosed in 2 cm 2 leaf chamber exhibited negative apparent Ci values at highest An. For the same leaves measured with the entire leaf chamber enclosed in the polyvinyl fluoride bag, Ci and An increased monotonically. While the measurements without the bag could be corrected for diffusion leaks, the required correction in An and transpiration rates was 100-500%, and there was large uncertainty in Farquhar et al. model parameters derived from 'corrected' An/Ci response curves because of uncertainties in true diffusion leaks. These data demonstrate th...
In this study we examined ecosystem respiration (RECO) data from 104 sites belonging to FLUXNET, the global network of eddy covariance flux measurements. The goal was to identify the main factors involved in the variability of RECO: temporally and between sites as affected by climate, vegetation structure and plant functional type (PFT) (evergreen needleleaf, grasslands, etc.). We demonstrated that a model using only climate drivers as predictors of RECO failed to describe part of the temporal variability in the data and that the dependency on gross primary production (GPP) needed to be included as an additional driver of RECO. The maximum seasonal leaf area index (LAIMAX) had an additional effect that explained the spatial variability of reference respiration (the respiration at reference temperature Tref=15 °C, without stimulation introduced by photosynthetic activity and without water limitations), with a statistically significant linear relationship (r2=0.52, P<0.001, n=104) even within each PFT. Besides LAIMAX, we found that reference respiration may be explained partially by total soil carbon content (SoilC). For undisturbed temperate and boreal forests a negative control of total nitrogen deposition (Ndepo) on reference respiration was also identified. We developed a new semiempirical model incorporating abiotic factors (climate), recent productivity (daily GPP), general site productivity and canopy structure (LAIMAX) which performed well in predicting the spatio‐temporal variability of RECO, explaining >70% of the variance for most vegetation types. Exceptions include tropical and Mediterranean broadleaf forests and deciduous broadleaf forests. Part of the variability in respiration that could not be described by our model may be attributed to a series of factors, including phenology in deciduous broadleaf forests and management practices in grasslands and croplands.
Abstract. Soil respiration is the sum of respiration processes in the soil and is a major flux in the global carbon cycle. It is usually assumed that the CO 2 efflux is equal to the soil respiration rate. Here we challenge this assumption by combining measurements of CO 2 with high-precision measurements of O 2 . These measurements were conducted on different ecosystems and soil types and included measurements of air samples taken from the soil profile of three Mediterranean sites: a temperate forest and two alpine forests. Rootfree soils from the alpine sites were also incubated in the lab. We found that the ratio between the CO 2 efflux and the O 2 influx (defined as apparent respiratory quotient, ARQ) was in the range of 0.14 to 1.23 and considerably deviated from the value of 0.9 ± 0.1 expected from the elemental composition of average plants and soil organic matter. At the Mediterranean sites, these deviations are explained as a result of CO 2 dissolution in the soil water and transformation to bicarbonate ions in these high-pH soils, as well as by carbonate mineral dissolution and precipitation processes. Thus, a correct estimate of the short-term, chamber-based biological respiratory flux in such soils can only be made by dividing the measured soil CO 2 efflux by the average (efflux-weighted) soil profile ARQ. Applying this approach to a semiarid pine forest resulted in an estimated short-term biological respiration rate that is 3.8 times higher than the chamber-measured surface CO 2 . The ARQ values often observed in the more acidic soils were unexpectedly low (< 0.7). These values probably result from the oxidation of reduced iron, which has been formed previously during times of high soil moisture and local anaerobic conditions inside soil aggregates. The results reported here provide direct quantitative evidence of a large temporal decoupling between soil-gas exchange fluxes and biological soil respiration.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.