<p>Exposure of all nine cell lines to scalar concentrations of ADC for a total of 3 days. IC50 dose response curves of SYD985, T-DM1 and ADC isotype control in all 9 primary USC cell lines with differential HER2 expression tested in vitro (i.e., three with HER2 3+ expression, two with HER2 2+ expression and four with HER2 1+ expression) at 3 days.</p>
<p>Exposure of all nine cell lines to scalar concentrations of ADC for a total of 6 days. IC50 dose response curves of SYD985, T-DM1 and ADC isotype control in all 9 primary USC cell lines with differential HER2 expression tested in vitro (i.e., three with HER2 3+ expression, two with HER2 2+ expression and four with HER2 1+ expression) at 6 days (ie, 144 hrs).</p>
<p>Exposure of all nine cell lines to scalar concentrations of ADC for a total of 6 days. IC50 dose response curves of SYD985, T-DM1 and ADC isotype control in all 9 primary USC cell lines with differential HER2 expression tested in vitro (i.e., three with HER2 3+ expression, two with HER2 2+ expression and four with HER2 1+ expression) at 6 days (ie, 144 hrs).</p>
<p>Table S1. Summary of status of tumors used in cell line and patient-derived xenograft models. Table S2. SYD985 and T-DM1 IC50, 95% confidence interval and % efficacy values from the in vitro cytotoxicity assays as depicted in Figure 1B. Table S3. Pharmacokinetic parameters for ADC (SYD985 and T-DM1) in BT-474-tumor-bearing mice after a single i.v. bolus injection of ADC. Figure S1. In vitro cytotoxicity. Figure S2. A-G HER2 expression in BT-474. Figure S3. HER2 cell surface expression quantified on a panel of 8 cell lines using the DAKO Qifikit. Figure S4. In vitro cytotoxicity. Figure S5. In vitro bystander killing. Figure S6. Mean ADC (SYD985 conjugated antibody) concentrations in plasma in carboxylesterase CES1c knock-out (-/-) , CES1c heterozygeous (+/-) and CES1c wild type (+/+) mice, after single i.v. bolus injection of SYD985 at 5 mg/kg (+/- SEM, n = 3).</p>
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