The main effectors in the innate immune system of Bombyx mori L. are antimicrobial peptides (AMPs). Here, we infected B. mori with varied inoculum sizes of Pseudomonas aeruginosa ATCC 25668 cells to investigate changes in morpho-anatomical responses, physiological processes and AMP production. Ultraviolet-visible spectra revealed a sharp change in λ max from 278 to 285 nm (bathochromic shift) in the hemolymph of infected B. mori incubated for 24 h. Further, Fourier Transform InfraRed studies on the hemolymph extracted from the infected B. mori showed a peak at 1550 cm −1 , indicating the presence of α-helical peptides. The peptide fraction was obtained through methanol, acetic acid and water mixture (90:1:9) extraction, followed by peptide purification using Reverse Phase High Performance Liquid Chromatography. The fraction exhibiting antibacterial properties was collected and characterized by Matrix-Assisted Laser Desorption/Ionization-Time of Flight. A linear α-helical peptide with flexible termini (LLKELWTKMKGAGKAVLGKIKGLL) was found, corresponding to a previously described peptide from ant venom and here denominated as Bm-ponericin-L1. The antibacterial activity of Bm-ponericin-L1 was determined against ESKAPE pathogens. Scanning electron microscopy confirmed the membrane disruption potential of Bm-ponericin-L1. Moreover,
Schiff base ligands or compounds are useful in modern inorganic chemistry.
Numerous transition metal-based catalysts have been synthesized with Schiff base
scaffolds. The application of such Schiff bases is also found in biological studies.
Herein, we have discussed the various synthetic procedures of diversified Schiff base
compounds and their metal complexes. The biological activity of those complexes has
also been delineated in this chapter with special emphasis. Various metal complexes
[Co(II), Ni(II), Cu(II), Zn(II) and Fe(III)] with different Schiff base compounds
displayed anti-fungal activity. Similarly, anti-viral activity was seen with Co(II) and
Pd(II) metal complexes. Many Schiff base-metal complexes are found, which showed
anti-cancer activity against various carcinoma cells like HpG2, MCF-7, A549,
HCT116, Caco-2 and PC-3. Similarly, the transition metal complexes (generally 1st and
2
nd row) of Schiff bases also exhibited good anti-bacterial activity against various
bacterial strains. The ionic-liquid-tagged Schiff bases have also been found to be good
anti-microbial agents.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.