Chicken embryo testThe destruction and detoxification of aflatoxins B,, G,, B, and G2 (50 &mL in 4% dimethyl sulfoxide) with ozone were confirmed by thinlayer chromatography and chicken embryo assay. Aflatoxins B1 and G, were sensitive to ozone and easily degraded with 1.1 mgiL of ozone within 5 min at room temperature. The result of Ames test showed the inactivation of the mutagenic activities of the toxins by ozone. Also, the deleterious acute effect of ozone-treated aflatoxin B, was not detected in the rat. On the other hand, aflatoxins B2 and G2 were rather resistant to ozone, requiring SO-60 min to degrade them completely with 34.3 mg/L of ozone.
An analytical method for determining proanthocyanidins by HPLC-electrochemical detection was developed, and the effect of free radicals on chill haze formation in beer was studied. The determination limits of procyanidin B3, catechin, and epicatechin by this analytical method were 30,10, and 10 pg/ L, respectively. Addition of H202 and irradiation with 6oCo y-rays accelerated the degradation of the proanthocyanidins and formation of chill haze in beer, while the addition of a metal scavenger, a H202
The lipase (EC. 3.1.1.3) of Pseudomonasfragi 22.39 B was purified by acidification of the culture supernatant, ammonium sulfate fractionation, and column chromatography on DEAEToyopearl 650m and DEAE-Sepharose CL-6B. The recovery of activity was about 48%. The purified lipase was homogeneous electrophoretically and its molecular weight was 33,000. The optimum pH and temperature for hydrolysis of olive oil emulsion were 9.0 and 65°C, respectively. The enzymewas stableupto 51°C atpH 9.0 for 24hr and in apH range from 6.5 to 10.5 at 30°C for 24hr. The lipase was inhibited by Zn2+, Fe2+, Fe3 + and cationic surfactants such as cetyltrimethylammonium bromide.
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