In order to show which of the initial lactic acid bacteria (LAB) contaminants are also 3 causing spoilage of a modified-atmosphere-packaged (MAP), marinated broiler leg product at 6ºC, 4 LAB were enumerated and identified on the 2 nd and 17 th day following manufacture. A total of 8 5 fresh and 13 spoiled packages were studied for LAB levels. In addition, aerobic mesophilic bacteria 6and Enterobacteriaceae were determined. The average CFU/g values in the 8 fresh packages were 71.3 × 10 3 , 9.8 × 10 3 and 2.6 × 10 2 on de Man Rogosa Sharpe agar (MRS), Plate Count Agar (PCA) 8and Violet Red Bile Glucose agar (VRBG), respectively. The commercial shelf life for the product 9 had been set as 12 days and all packages analyzed on the 17 th day were deemed unfit for human 10 consumption by the sensory analysis. The corresponding CFU/g averages in the spoiled product 11 were 1.4 × 10 9 , 1.1 × 10 9 and 3.9 × 10 7 on MRS, PCA and VRBG agar, respectively. For 12 characteriza tion of LAB population, 104 colonies originating from the fresh and 144 colonies from 13 the spoiled packages were randomly picked, cultured pure and identified to species level using a 16 14 and 23S rDNA HindIII RFLP (ribotyping) database. The results showed that enterococci (35.7% of 15 the LAB population) were dominating in the fresh product whereas carnobacteria (59.7%) 16 dominated among the spoilage LAB. Enterococcus faecalis, Carnobacterium piscicola and 17Carnobacterium divergens were the main species detected. In general, when the initial LAB 18 population is compared to the spoilage LAB, a shift from homofermentative cocci towards 19 carnobacteria, Lactobacillus sakei/curvatus and heterofermentative rods is seen in this marinated 20 product. 21 22 3
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