. Significance: Hyperspectral imaging (HSI) provides rich spectral information for improved histopathological cancer detection. However, acquiring high-resolution HSI data for whole-slide imaging (WSI) can be time-consuming and requires a huge amount of storage space. Aim: WSI using a color camera can be achieved with fast speed, high image resolution, and excellent image quality due to the established techniques. We aim to develop an RGB-guided unsupervised hyperspectral super-resolution reconstruction method that is hypothesized to improve image quality while maintaining the spectral characteristics. Approach: High-resolution hyperspectral images of 32 histologic slides were obtained via automated WSI. High-resolution RGB histology images were registered to the hyperspectral images for RGB guidance. An unsupervised super-resolution network was trained to take the downsampled low-resolution hyperspectral patches (LR-HSI) and high-resolution RGB patches (HR-RGB) as inputs to reconstruct high-resolution hyperspectral patches (HR-HSI). Then, an Inception-based network was trained with the HR-RGB, original HR-HSI, and generated HR-HSI, respectively, for whole-slide histopathological cancer detection. Results: Our super-resolution reconstruction network generated high-resolution hyperspectral images with well-maintained spectral characteristics and improved image quality. Image classification using the original hyperspectral data outperformed RGB because of the extra spectral information. The generated hyperspectral image patches further improved the results. Conclusions: The proposed method potentially reduces image acquisition time, saves storage space without compromising image quality, and improves the image classification performance.
. Significance Spectral imaging, which includes hyperspectral and multispectral imaging, can provide images in numerous wavelength bands within and beyond the visible light spectrum. Emerging technologies that enable compact, portable spectral imaging cameras can facilitate new applications in biomedical imaging. Aim With this review paper, researchers will (1) understand the technological trends of upcoming spectral cameras, (2) understand new specific applications that portable spectral imaging unlocked, and (3) evaluate proper spectral imaging systems for their specific applications. Approach We performed a comprehensive literature review in three databases (Scopus, PubMed, and Web of Science). We included only fully realized systems with definable dimensions. To best accommodate many different definitions of “compact,” we included a table of dimensions and weights for systems that met our definition. Results There is a wide variety of contributions from industry, academic, and hobbyist spaces. A variety of new engineering approaches, such as Fabry–Perot interferometers, spectrally resolved detector array (mosaic array), microelectro-mechanical systems, 3D printing, light-emitting diodes, and smartphones, were used in the construction of compact spectral imaging cameras. In bioimaging applications, these compact devices were used for in vivo and ex vivo diagnosis and surgical settings. Conclusions Compact and ultracompact spectral imagers are the future of spectral imaging systems. Researchers in the bioimaging fields are building systems that are low-cost, fast in acquisition time, and mobile enough to be handheld.
Hyperspectral imaging is a label-free and non-invasive imaging modality that seeks to capture images in different wavelengths. In this study, we used a vision transformer that was pre-trained from video data to detect thyroid cancer on hyperspectral images. We built a dataset of 49 whole slide hyperspectral images (WS-HSI) of thyroid cancer. To improve training, we introduced 5 new data augmentation methods that transform spectra. We achieved an F-1 score of 88.1% and an accuracy of 89.64% on our test dataset. The transformer network and the whole slide hyperspectral imaging technique can have many applications in digital pathology.
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