Purpose:This study aimed to investigate the effects of microRNA-222-3p on activated B cell-like-type diffuse large B-cell lymphoma cells and the regulatory relationship between microRNA-222-3p and phosphatase 2 regulatory subunit B alpha.Method:The expression of microRNA-222-3p was detected in activated B cell-like-type diffuse large B-cell lymphoma tissues and cells by quantitative reverse transcription polymerase chain reaction. The regulatory effects of microRNA-222-3p on the proliferation, invasion, and apoptosis of activated B cell-like-type diffuse large B-cell lymphoma cells were analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), colony formation, flow cytometry, and Transwell assay. The regulatory relationship between microRNA-222-3p and phosphatase 2 regulatory subunit B alpha was determined by luciferase reporter gene and RNA pull-down assay. In addition, the effects of microRNA-222-3p on tumor growth were further analyzed in mice.Results:MicroRNA-222-3p and phosphatase 2 regulatory subunit B alpha were significantly up- and downregulated in activated B cell-like-type diffuse large B-cell lymphoma tissues and cells, respectively. Phosphatase 2 regulatory subunit B alpha was a target of microRNA-222-3p. MicroRNA-222-3p promoted the proliferation and invasion and inhibited the apoptosis of activated B cell-like-type diffuse large B-cell lymphoma cells. Phosphatase 2 regulatory subunit B alpha reversed the tumor-promoting effects of microRNA-222-3p on activated B cell-like-type diffuse large B-cell lymphoma cells. In addition, microRNA-222-3p promoted the tumor growth in mice and downregulated phosphatase 2 regulatory subunit B alpha in tumor tissues.Conclusion:MicroRNA-222-3p promoted the proliferation and invasion and inhibited the apoptosis of activated B cell-like-type diffuse large B-cell lymphoma cells through suppressing phosphatase 2 regulatory subunit B alpha expression.

Background This study aimed to investigate the mechanism of microRNA-222-3p (miR-222-3p) on the progression of diffuse large B-cell lymphoma (DLBCL) cells.Methods DLBCL tissue was isolated from DLBCL patients during surgery. OCI-LY10 and U2932 cells were cultured. Then, qRT-PCR, Western blot, luciferase reporter gene assay, RNA pull-down assay, MTT assay, colony formation analysis, flow cytometry as well as Transwell assay were used to observe the effect of miR-222-3p on proliferation, migration, invasion and apoptosis of DLBCL cells. Furthermore, the tumor growth affected by miR-222-3p was further investigated based on animal experiment.Results Compared with the control group, the expression level of miR-222-3p was up-regulated in DLBCL group. The luciferase reporter gene and RNA pull down assay showed that PPP2R2A 3’-untranslated region (3’-UTR) carried the directly binding site of miR-222-3p. Furthermore, MTT assay, colony formation, qRT-PCR and Western blot showed that miR-222-3p promoted the DLBCL cell proliferation and invasion, and inhibited apoptosis. Finally, the mice experiment showed that miR-222-3p mimics inhibited PPP2R2A expression and promoted tumor growth in vivo.Conclusions Upregulation of miR-222-3p might take part in the progression of DLBCL by suppressing PPP2R2A expression. Furthermore, miR-222-3p promoted the DLBCL cell proliferation and invasion, and inhibited apoptosis.