Yarrowia lipolytica, an oleaginous yeast with the GRAS status, has been
developed as a platform for the chemical production. Specifically,
promoter engineering is an important approach to regulating gene
expression at the transcriptional level, which is of great significance
in constructing microbial cell factories. Until now, some work on the
promoter study has been carried out in Y. lipolytica. However, compared
with other microorganisms, such as Saccharomyces cerevisiae, Bacillus
subtilis, and etc., obtained achievements still need further
replenishment and development for Y. lipolytica. For this, we screened
81 endogenous promoters in Y. lipolytica, mainly involved in carbon
metabolism, amino acid metabolism, and lipid metabolism. As a result, we
obtained 15 strong promoters, 41 medium strength promoters, and 25 weak
promoters, with the strength spanning from 0.06% to 1.60-fold of PTEF
promoter. In general, our study provides a unique and available
endogenous promoter library for Y. lipolytica.
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