Background
Cervical cancer is the most fatal gynecological carcinoma in the world. It is urgent to explore novel prognostic biomarkers and intervention targets for cervical cancer.
Methods
Through integrated quantitative proteomic strategy, we investigated the protein expression profiles of cervical cancer; 28 fresh frozen tissue samples (11 adenocarcinoma (AC), 12 squamous cell carcinoma (SCC) and 5 normal cervixes (HC)) were included in discover cohort; 45 fresh frozen tissue samples (19 AC, 18 SCC and 8 HC) were included in verification cohort; 140 paraffin-embedded tissues samples of cervical cancer (85 AC and 55 SCC) were used for immunohistochemical evaluation (IHC) of coatomer protein subunit alpha (COPA) as a prognostic biomarker for cervical cancer; how deficiency of COPA affects cell viability and tumorigenic ability of cervical cancer cells (SiHa cells and HeLa cells) were evaluated by cell counting kit-8 and clone formation in vitro.
Results
We identified COPA is a potential prognostic biomarker for cervical cancer in quantitative proteomics analysis. By retrospective IHC analysis, we additionally verified the proteomics results and demonstrated moderate or strong IHC staining for COPA is an unfavourable independent prognostic factor for cervical cancer. We also identified COPA is a potential pharmacological intervention target of cervical cancer by a series of in vitro experiments.
Conclusion
This study is the first to demonstrate that COPA may contribute to progression of cervical cancer. It can serve as a potential prognostic biomarker and promising intervention target for cervical cancer.
Fluorescence materials have been widely employed for anti-counterfeiting techniques owing to their high-throughput, facile identification, and simplicity of production. However, the stability of the materials is a prerequisite for their subsequent application. Here, a series of SrGa12O19: Sm3+, Tb3+ phosphors with multi-color luminescence are obtained successfully by the traditional solid-state method. These Sm3+/Tb3+ co-doped phosphors emit green, orange, and yellow-green under the excitation of 254, 365, and 254 nm+365 nm ultraviolet (UV) lamps, respectively. After removal of the UV lamp, the green long persistent luminescence (LPL) phenomenon is exhibited and then vanishes 15 s later. The dynamic PL and LPL are associated with the interaction between PL and trapping centers. Notably, as-obtained phosphors show excellent stability against both air, water, and high temperature, which makes the as-obtained phosphors a great application potential in high-level anti-counterfeiting with high stability.
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