The interaction of the single azo dye, carmine with fish-sperm dsDNA is inspected in pH 3.2 H2SO4with electrochemical method on the surface of nanometer TiO2modified carbon paste electrode. After the addition of dsDNA, the peak currents of oxidation and reduction peaks of carmine decrease with a positive shift of potential, indicating that intercalation interaction between the dye and dsDNA is taken place. This is consistent with fluorescence spectra results. The binding constant and binding ratio is calculated as 4.92×108and 1:2, respectively. Furthermore, the decrease in the oxidation peak currents is found proportional to dsDNA concentration in the range of 21.24-127.44 μg·mL-1 with a detection limit of 16.04 μg•mL-1.
A poly silk peptide film pH sensor has been developed using zero current potentiometry system. A poly silk peptide film coated pencil graphite electrode is connected in series between the working and counter electrodes of a potentiostat, and immersed in solution together with a reference electrode. When the solution pH varies, the resulting zero current potentiometry is linear with the values of the solution pH in the range of 1.81 to 11.58. This pH sensor shows high stability, accuracy, selectivity and reproduction.
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