Unisexual and heterosexual reproduction in the pathogenic yeast Cryptococcus neoformans enables de novo phenotypic and genotypic plasticity with frequent aneuploidy and rapid adaptation.

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A long and ever-expanding roster of small (∼20–30 nucleotides) RNAs has emerged during the last decade, and most can be subsumed under the three main headings of microRNAs(miRNAs), Piwi-interacting RNAs(piRNAs), and short interferingRNAs(siRNAs). Among the three categories, miRNAs is the most quickly expanded group. The most recent number of identified miRNAs is 16,772 (Sanger miRbase, April 2011). However, there are insufficient publications on their primary forms, and no tissue-specific small RNAs precursors have been reported in the epididymis. Here, we report the identification in rats of an epididymis-specific, chimeric, noncoding RNA that is spliced from two different chromosomes (chromosomes 5 and 19), which we named HongrES2. HongrES2 is a 1.6 kb mRNA-like precursor that gives rise to a new microRNA-like small RNA (mil-HongrES2) in rat epididymis. The generation of mil-HongrES2 is stimulated during epididymitis. An epididymis-specific carboxylesterase named CES7 had 100% cDNA sequence homology at the 3′end with HongrES2 and its protein product could be downregulated by HongrES2 via mil-HongrES2. This was confirmed in vivo by initiating mil-HongrES2 over-expression in rats and observing an effect on sperm capacitation.

We propose a new activity on verification and validation (V&V) of MHD codes presently employed by the fusion community as a predictive capability tool for liquid metal cooling applications, such as liquid metal blankets. The important steps in the development of MHD codes starting from the 1970s are outlined first and then basic MHD codes, which are currently in use by designers of liquid breeder blankets, are reviewed. A benchmark database of five problems has been proposed to cover a wide range of MHD flows from laminar fully developed to turbulent flows, which are of interest for fusion applications: (A) 2D fully developed laminar steady MHD flow, (B) 3D laminar, steady developing MHD flow in a non-uniform magnetic field, (C) quasitwo-dimensional MHD turbulent flow, (D) 3D turbulent MHD flow, and (E) MHD flow with heat transfer (buoyant convection). Finally, we introduce important details of the proposed activities, such as basic V&V rules and schedule. The main goal of the present paper is to help in establishing an efficient V&V framework and to initiate benchmarking among interested parties. The comparison results computed by the codes against analytical solutions and trusted experimental and numerical data as well as code-to-code comparisons will be presented and analyzed in companion paper/papers.

Numerical simulations of liquid metal (LM) magnetohydrodynamic (MHD) flows in a prototypical dual coolant lead lithium (DCLL) blanket module have been performed under conditions relevant to a real LM blanket system, i.e. strong magnetic field of 5 T (Hartmann number Ha ~10 4 ) and poloidal flow velocity of 0.1 m s −1 (Reynolds number Re ~10 5 ). The proposed blanket model includes all essential sub-components of a real LM blanket: radial supply ducts, inlet and outlet manifolds, multiple poloidal ducts and a U-turn zone. The computations were performed using a DNS-like finite-volume code on a very fine mesh of ~320 × 10 6 cells to accurately capture all flow features. Four cases have been simulated, including: Case 1 for electrically conducting walls, Case 4 for non-conducting walls, and Cases 2 and 3 with partial SiC FCIs electrical insulation of the blanket conduits using silicon carbide flow channel inserts placed at selected locations. In these four cases, the computed MHD flows were analyzed for: (1) MHD pressure drop, (2) flow distribution, (3) flows in particular blanket components, (4) 3D and flow development effects, and (5) unsteady flows. Many important conclusions have been made, regarding effectiveness of the FCI to reduce the MHD pressure drop, flow balancing in the parallel channels, characteristic flow patterns in all blanket sub-components, electromagnetic coupling between the flows, 3D MHD effects, flow development length, and unsteady effects. This study is limited to purely MHD flows. Next study will focus on a DCLL blanket with a volumetric heating.

Background: Epididymis displays decreased proliferation during postnatal development. Results: miR-29a and nuclear autoantigenic sperm protein (NASP) were dramatically up-regulated and down-regulated, respectively, during postnatal epididymal development. Moreover, miR-29a can inhibit epididymal cell proliferation by targeting Nasp in vitro. Conclusion: miR-29a may inhibit epididymal epithelial cell proliferation during postnatal epididymal development by targeting Nasp. Significance: miR-29a may be necessary for epididymal maturation.