The study analysed healthcare workers' (HCWs) knowledge, practices, and attitudes regarding coronavirus disease 2019 . A cross-sectional survey was conducted from February 4 th to February 8 th , 2020, involving a total of 1357 HCWs across 10 hospitals in Henan, China. Of those surveyed, 89% of HCWs had sufficient knowledge of COVID-19, more than 85% feared self-infection with the virus, and 89.7% followed correct practices regarding COVID-19. In addition to knowledge level, some risk factors including work experience and job category influenced HCWs' attitudes and practice concerning COVID-19. Measures must be taken to protect HCWs from risks linked to job category, work experience, working hours, educational attainment, and frontline HCWs.
A colorimetric assay has been developed for parallel detection of Cd(2+), Ni(2+) and Co(2+) utilizing peptide-modified gold nanoparticles (P-AuNPs) as a sensing element based on its unique surface plasmon resonance properties. The functional peptide ligand, CALNNDHHHHHH, was self-assembled on gold nanoparticles (AuNPs) to produce P-AuNPs probe. The P-AuNPs probe could be used to simultaneously detect and showed different responses to the three ions Cd(2+), Ni(2+) and Co(2+) in an aqueous solution based on the aggregation-induced color change of AuNPs. The method showed good selectivity for Cd(2+), Ni(2+) and Co(2+) over other metal ions, and detection limit as low as 0.05 μM Cd(2+), 0.3 μM Ni(2+) or 2 μM Co(2+). To simultaneously (or parallel) detect the three metal ions coexisting in a sample, EDTA and imidazole were applied to mask Co(2+) and Ni(2+) for detecting Cd(2+), glutathione and EDTA were applied to mask Cd(2+) and Co(2+) for detecting Ni(2+), and glutathione and imidazole were applied to mask Cd(2+) and Ni(2+) for detecting Co(2+). Finally, the simple and cost-effective probe could be successfully applied for simultaneously detecting Cd(2+), Ni(2+), and Co(2+) in river water. Because this novel P-AgNPs-based probe design offers many advantages, including simplicity of preparation and manipulation compared with other methods that employ specific strategies, the sensing system shows potential application in the developing region for monitoring water quality.
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