TianQin is a proposal for a space-borne detector of gravitational waves in the millihertz frequencies. The experiment relies on a constellation of three drag-free spacecraft orbiting the Earth. Inter-spacecraft laser interferometry is used to monitor the distances between the test masses. The experiment is designed to be capable of detecting a signal with high confidence from a single source of gravitational waves within a few months of observing time. We describe the preliminary mission concept for TianQin, including the candidate source and experimental designs. We present estimates for the major constituents of the * experiment's error budget and discuss the project's overall feasibility. Given the current level of technology readiness, we expect TianQin to be flown in the second half of the next decade.

We present a realistic purification scheme for pure non-maximally entangled states. In the scheme, Alice and Bob at two distant parties first start with two shared but less entangled photon pairs to produce a conditional four-photon GHZ state, then perform a 45 • polarization measurement onto one of the two photons at each party such that the remaining two photons are projected onto a maximally entangled state. 03.65.Bz, 42.50.Ar

IntroductionIn guiding human embryonic stem cell (hESC) technology toward the clinic, 1 key issue to be addressed is a lack of standardization in the culture and maintenance of hESCs. In the absence of mouse embryonic fibroblast (MEF) feeder layers, many researchers rely on "conditioning" in which medium is first exposed to MEFs to acquire soluble factors that support the propagation of undifferentiated hESCs in culture. It has been difficult to discern how MEF conditioning enables hESCs to maintain an undifferentiated state. Other common features of more recently developed hESC culture conditions include the presence of fibroblast growth factor-2 (FGF2), the absence of serum, and the presence of a serum substitute such as KnockOut Serum Replacer (KSR, proprietary formulation; Invitrogen, Carlsbad, CA). [1][2][3] Other factors suggested to play a role in supporting the maintenance of hESCs include transforming growth factor ␤1 (TGF␤1), 4 activin A (ActA), 5,6 platelet-derived growth factor (PDGF) and sphingosine-1-phosphate, 7 BIO, a small-molecule inhibitor of GSK3␤, 8 and neurotrophins. 9 Several defined medium systems have been described for hESCs and are based upon FGF2 in combination with nodal, 10 TGF␤1, GABA, and pipecolic acid, plus lithium chloride, 11 Wnt3a plus April/BAFF, 12 or the N2/B27 supplements. 13 Although these studies have focused on identifying growth factors and conditions that support the proliferation of undifferentiated hESCs, little is known about the cell-surface receptors that are activated when hESCs are exposed to conditions favorable for self-renewal. A number of receptor tyrosine kinases (RTKs) are expressed at high levels on hESCs, 14 including insulin-like growth factor-1 receptor (IGF1R), fibroblast growth factor receptor (FGFR1), and EPHA1, as well as ERBB2 and ERBB3 (which are members of the epidermal growth factor receptor [EGFR] family), while expression of FGFR2 (EGFR) FGFR4, vascular endothelial growth factor receptor-2 (VEGFR2), IGFR2, KIT, and RET has also been detected. 15,16 RTKs are likely to be central signaling effectors 17 that influence survival, apoptosis, proliferation, or differentiation decisions in pluripotent cells. To determine if any of these RTKs are involved in self-renewal, we simultaneously interrogated the tyrosine phosphorylation status of 42 RTKs in hESCs grown in MEF-conditioned medium (CM) and developed a defined medium for hESC culture. The online version of this article contains a data supplement.The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked ''advertisement'' in accordance with 18 USC section 1734. For personal use only. on May 7, 2018. by guest www.bloodjournal.org From Materials and methods Cell cultureThe National Institutes of Health (NIH)-registered H1, BG01, BG02, and BG03 hESC lines, as well as CyT49, an hESC line isolated using human feeder cells under good manufacturing process (GMP) conditions (Novocell, San Diego, CA), we...