Purpose: The correlation of the hedgehog signaling pathway with the progression, prognosis, and therapeutics of intrahepatic cholangiocellular carcinoma (ICC) has not been well documented. The study aimed to investigate the expression, prognostic significance, and therapeutic value of hedgehog components in ICC.Experimental Design: Two independent cohorts of 200 patients with ICC were enrolled. By real-time PCR and immunohistochemistry assay, hedgehog components expression was evaluated. The prognostic values of hedgehog proteins were identified and verified. Cyclopamine or siRNA-targeting Gli was used to block the hedgehog signaling. Cell proliferation and apoptosis were observed by CCK8, cell cycle, and annexin V staining assays. In vivo murine tumor model was used to evaluate the role of hedgehog in ICC.Results: In ICC tissues, the Gli1 nuclear immune-intensity was associated with intrahepatic metastasis and the expression of Gli2 was associated with intrahepatic metastasis, venous invasion, and Unio Internationale Contra Cancrum (UICC) pT characteristics. In survival analysis, high Gli1 or Gli2 expressers had an unfavorable overall survival (OS) prognosis and a shorter disease-free survival (DFS) than those with low expression. In multivariate analysis, Gli1 expression was found to be an independent prognostic factor of OS, which was validated by another independent cohort. Furthermore, blocking the hedgehog signaling by cyclopamine or siRNA-targeting Gli1 resulted in apoptosis and growth inhibition in ICC cells.Conclusions: This study shows, for the first time, activation of hedgehog pathway associated with the progression and metastasis in ICC, which may provide prognostic and therapeutic values for this tumor.
Fire protective clothing worn by the emergency responders may be exposed to intensive heat condition time and time again during a firefighting work. In this research, the level of thermal protection retained by the fire protective clothing after repeated exposures to flash fire was investigated from bench-scale test to full-scale test. A thermal protective performance tester and an instrumented manikin with a transverse motion system device which was capable of simulating the action of running across the flame were used for the exposure test. Physical properties (mass, thickness, thermal shrinkage, tear strength) and thermal protective property of the test specimens were examined after each exposure. The results showed that repeated heat exposures resulted in continuous decrease of mechanical performance of the fabrics. The thermal protective performance of fabrics with good thermal dimensional stability such as polybenzimidazole/Kevlar and flame resistant cotton decreased after exposure. For the fabrics with severe thermal shrinkage such as Nomex IIIA and polysulfonamide, the thermal protective performance was improved due to the increase of fabric thickness induced by thermal shrinkage. However, this positive effect of thermal shrinkage diminished in the manikin test as it decreased the air gap size between the garment and flame manikin. The thermal protective property of Nomex IIIA garment exhibited continuous decrease after repeated exposures. This study was expected to provide new sights for the performance evaluation and application of fire protective clothing.
In this study, common flame-retardant fabrics were treated with single washing or abrasion and their interactions to simulate wearing away during use. The changes in thickness, mass/m and protective performance of the fabrics under both flame and radiation environments were evaluated. Results demonstrated that the protective performance was firstly increased after washing or abrasion, and then decreased with further increasing washing or abrasion cycles. After certain treatment cycles, the combined effect of washing and abrasion was significantly greater than the single effect of washing or abrasion alone. The interaction modes of washing and abrasion also showed significant differences in protective performance under a flame test. Under radiation exposure, the effect of combined washing and abrasion was more obvious. There was a positive correlation between the fabric weight and its protective performance with different treatments. The findings provide useful guidance for the actual use and maintenance of protective clothing.
In this work, a novel alginate/citrate composite aerogel (CA–SC) was synthesized by chemical grafting technology combined with vacuum freeze-drying method, and CA–SC was used for removing calcium (Ca2+) and magnesium (Mg2+) ions from water. The experimental results indicate that the as-prepared CA–SC has a high affinity for Ca2+ and Mg2+ and can remove 96.5% of Ca2+ (or 96.8% of Mg2+) from the corresponding solution. The maximum adsorption capacities of CA–SC for Ca2+ and Mg2+ are 62.38 and 36.23 mg/g, respectively. These values are higher than those of the most reported Ca2+-sorbents and Mg2+-sorbents. The CA–SC adsorbent can be regenerated through a simple pickling step, and its adsorption performance keeps stable after repeated use. Analysis of the adsorption mechanism shows that the CA–SC combines Ca2+ and Mg2+ in water mainly through coordination effect.
Photoconductor arrays with both high responsivity and large ON/OFF ratios are of great importance for the application of image sensors. Herein, a ZnO vertical nanorod array based photoconductor with a light absorption layer separated from the device channel has been designed, in which the photo-generated carriers along the axial ZnO nanorods drive to the external electrodes through nanorod-nanorod junctions in the dense layer at the bottom. This design allows us to enhance the photocurrent with unchanged dark current by increasing the ratio between the ZnO nanorod length and the thickness of the dense layer to achieve both high responsivity and large ON/OFF ratios. As a result, the as-fabricated devices possess a high responsivity of 1.3 × 105 A/W, a high ON/OFF ratio of 790, a high detectivity of 1.3 × 1013 Jones, and a low detectable light intensity of 1 μW/cm2. More importantly, the developed approach enables the integration of ZnO vertical nanorod array based photodetectors as image sensors with uniform device-to-device performance.
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