a b s t r a c tWhile hydraulic fracturing has revolutionized hydrocarbon production from unconventional resources, waterless or reduced-water fracturing technologies have been actively sought due to concerns arising from the heavy use of water. This study investigates the feasibility of fracture stimulation by using cryogenic fluids to create a strong thermal gradient generating local tensile stress in the rocks surrounding a borehole. Cracks form when the tensile stress exceeds the material's tensile strength. This mechanism has not been exploited in the context of stimulation and may be used to fracture reservoir rocks to reduce or eliminate water usage. This paper reports initial results from a laboratory study of cryogenic fracturing. In particular, we have developed experimental setups and procedures to conduct cryogenic fracturing tests with and without confining stress, with integrated cryogen transport, measurements, and fracture characterization. Borehole pressure, liquid nitrogen, and temperature can be monitored continuously. Acoustic signals are used to characterize fractures before and after the experiments. Cryogenic tests conducted in the absence of the confining stress were able to create cracks in the experimental blocks and alter rock properties. Fractures were created by generating a strong thermal gradient in a concrete block semi-submerged in liquid nitrogen. Increasing the number of cryogenic stimulations enhanced fracturing by both creating new cracks as well as widening the existing cracks. By comparing the cryogenic fracturing results from unstressed weak concrete and sandstone, we found that the generation of fractures is dependent on the material properties. Water in the formation expands as it freezes and plays a competing role during cryogenic cooling with rock contraction, thus is an unfavorable factor. A rapid cooling rate is desired to achieve high thermal gradient.
Background Probiotic lactic acid bacteria (LAB) support a functional and balanced immune system, and contribute to immune modulatory effects in combatting microbial pathogens, including viruses. Most cervical cancers are associated with anogenital region infection with high-risk (HR) human papillomavirus (HPV). In this study, we analyzed the antiviral activity of Bifidobacterium adolescentis SPM1005-A in the SiHa cervical cancer cell line expressing HPV type 16. Methods We assessed the cellular toxicity of B. adolescentis SPM1005-A in SiHa cells by the Trypan blue dye exclusion assay. Cells (3.6 × 10 5 ) in culture plates with or without B. adolescentis SPM1005-A in the same type of medium, were incubated with HPV type 16 at a concentration of 5.1 × 10 7 cfu/ml. For antiviral analysis, we performed quantitative real-time PCR (qRT-PCR) for E6 and E7 oncogene expressions and observed protein levels by immunoblotting. Results The qRT-PCR results showed that E6 and E7 mRNA levels decreased simultaneously. Western blot analysis revealed that the E6 protein expression slightly decreased after 24 and 48 h, but the level of E7 protein expression appear unaffected compared with that in the control. Decreased HPV16 E6 and E7 mRNA transcript and protein levels were not associated with cell morphology or significant cytotoxic effects. Conclusions This study showed that B. adolescentis SPM1005-A had antiviral activity through suppression E6 and E7 oncogene expression. The results suggest that B. adolescentis SPM1005-A could be potential applications of HPV-associated cervical cancer prevention.
During the past two decades, hydraulic fracturing has significantly improved oil and gas production from shale and tight sandstone reservoirs in the United States and elsewhere.Considering formation damage, water consumption, and environmental impacts associated with water-based fracturing fluids, efforts have been devoted to developing waterless fracturing technologies because of their potential to alleviate these issues. Herein, key theories and features of waterless fracturing technologies, including Oil-based and CO 2 energized oil fracturing, explosive and propellant fracturing, gelled LPG and alcohol fracturing, gas fracturing, CO 2 fracturing, and cryogenic fracturing, are reviewed. We then experimentally elaborate on the efficacy of liquid nitrogen in enhancing fracture initiation and propagation in concrete samples, and shale and sandstone reservoir rocks. In our laboratory study, cryogenic fractures generated were qualitatively and quantitatively characterized by pressure decay tests, acoustic measurements, gas fracturing, and CT scans. The capacity and applicability of cryogenic fracturing using liquid nitrogen are demonstrated and examined. By properly formulating the technical procedures for field implementation, cryogenic fracturing using liquid nitrogen could be an advantageous option for fracturing unconventional reservoirs.
Field data suggest that stress level and joint condition affect shear-wave propagation in jointed rock masses. However, the study of long-wavelength propagation in a jointed rock mass is challenging in the laboratory, and limited data are available under controlled test conditions. Long-wavelength P-wave and S-wave propagation normal to joints, using an axially loaded jointed column device, reproduces a range of joint conditions. The effects of the normal stress, loading history, joint spacing, matched surface topography (i.e., joint roughness), joint cementation (e.g., after grouting), joint opening, and plasticity of the joint filling on the P-wave and S-wave velocities and on S-wave attenuation are notable. The ratio [Formula: see text] in jointed rock masses differs from that found in homogeneous continua. The concept of Poisson’s ratio as a function of [Formula: see text] is unwarranted, and [Formula: see text] can be interpreted in terms of jointed characteristics. Analytic models that consider stress-dependent stiffness and frictional loss in joints as well as stress-independent properties of intact rocks can model experimental observations properly and extract joint properties from rock-mass test data. Thus, joint properties and normal stress have a prevalent role in propagation velocity and attenuation in jointed rock masses.
The small-strain shear modulus depends on stress in uncemented soils. In effect, the shear-wave velocity, which is often used to calculate shear stiffness, follows a power equation with the mean effective stress in the polarization plane V s 5 aðs m 9 =1 kPaÞ b , where the a factor is the velocity at 1 kPa, and the b exponent captures the velocity sensitivity to the state of stress. The small-strain shear stiffness, or velocity, is a constant-fabric measurement at a given state of stress. However, parameters a and b are determined by fitting the power equation to velocity measurements conducted at different effective stress levels, so changes in both contact stiffness and soil fabric are inherently involved. Therefore, the a and b parameters should be linked to soil compressibility C C. Compiled experimental results show that the a factor decreases and the b exponent increases as soil compressibility C C increases, and there is a robust inverse relationship between a and b for all sediments: b 0:73 2 0:27 log½a=ðm=sÞ. Velocity data for a jointed rock mass show similar trends, including a power-type stress-dependent velocity and inverse correlation between a and b; however, the a-b trend for jointed rocks plots above the trend for soils.
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