Background Radiofrequency (RF) oscillations generate thermal tissue reactions, the patterns of which vary depending on the mode and efficiency of energy delivery. The aim of our study was to analyze patterns of RF-induced thermal tissue reactions according to the modes of RF delivery, including continuous and gated modes, using an alternating current, invasive bipolar RF device. Methods RF energies at frequencies of 1 and 2 MHz were delivered at respective experimental settings into ex vivo bovine liver tissue at a 0.5-mm microneedle penetration depth. The tissue samples were then evaluated thermometrically. A histologic study was performed to evaluate RF-induced thermal tissue reactions at a 3.0-mm microneedle penetration depth. Results Thermal imaging study revealed homogenous, well-demarcated, square-shaped zones of RF-induced thermal reactivity on the treated area. Multivariate linear regression analysis revealed that higher temperature elevations immediately after RF treatment (∆T 1 ) were positively associated with RF frequency, power, conduction time/pulse pack, and off-time between pulse packs and negatively associated with total off time. In the 1-MHz experimental setting, higher ∆T 1 showed a positive association with power, conduction time/pulse pack, and off-time between pulse packs and a negative association with the number of pulse packs. In the 2-MHz setting, however, higher ∆T 1 was positively associated with only total treatment time. Conclusion Thermometric effects during bipolar and gated RF treatments are significantly associated with the frequency, power, and pulse widths and cycles of pulse packs.
Background: Various laser-and light-based devices have been introduced as complementary or alternative treatment modalities for dermatophytosis, particularly for finger or toenail onychomycosis.Objective: This study aimed to comparatively evaluate the antifungal effects of 405-nm and 635-nm dual-band diode lasers using an in vivo guinea pig model of dermatophytosis. Materials and Methods: A guinea pig model was developed by the repetitive application of fungal spore preparations to the back skin of guinea pigs. Dual-diode laser treatment was delivered to the guinea pig skin at a power of 24 mW at a wavelength of 405 nm and 18 mW at 635 nm for 12 min. The treatments were administered three times weekly for 2 weeks, and a mycological study was performed. Results: Mycological studies using scraped samples obtained from treatment groups A (N = 8) and B (N = 8) after dual-diode laser treatment revealed that seven of eight (87.5%) samples in each group had negative results for direct potassium hydroxide microscopy and fungal culture studies. Skin specimens from each infected laser-untreated guinea pig exhibited spongiotic psoriasiform epidermis with parakeratosis. Meanwhile, skin specimens from infected laser-treated guinea pigs in groups A and B demonstrated thinner epidermal thickness than those from infected untreated controls but thicker than those from uninfected treated controls without noticeable inflammatory cell infiltration in the dermis. Conclusion:The guinea pig dermatophytosis model can be used to comparatively evaluate the efficacy and safety of various treatment modalities, including dual-diode lasers, for superficial fungal skin infection.
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