The purpose of this study was to determine the test–retest reliability of the International Physical Activity Questionnaire (IPAQ) for older adults in Serbia. Six hundred and sixty older adults (352 men, 53%; 308 women, 47%; mean age 67.65±5.76 years) participated in the study. To examine test–retest reliability, the participants were asked to complete the IPAQ on two occasions 2 weeks apart. Moderate reliability was observed between the repeated IPAQ, with intraclass correlation coefficients ranging from 0.53 to 0.91. The least reliability was established in leisure time activity (0.53) and the most reliability in the transport domain (0.91). Men and women had similar intraclass correlation coefficients for total physical activity (0.71 versus 0.74, respectively), while the biggest difference was obtained for housework in men (0.68) and in women (0.90). Our study shows that the long version of the IPAQ is a reliable instrument for assessing physical activity levels in older adults and that it may be useful for generating internationally comparable data.

[Purpose] The purpose of this study was to determine the effects of short-term exercise training on the cardiorespiratory fitness (CRF) of individuals suffering from myocardial infarction. [Subjects] A total of 60 participants were divided into experimental (EXP; n=30, mean age 56.7 ± 2.8 years, body mass 80.7 ± 10.7 kg, body height 171.9 ± 7.2 cm) and control (CON; n=30, mean age 56.5 ± 3.1 years, body mass 84.4 ± 12.4 kg, body height 171.5 ± 12.4 cm) groups. [Methods] The members of the EXP group took part in an organized daily physical exercise program (Monday through Sunday), for a period of 3 weeks. The exercise program consisted of 60 min daily specialized fitness exercises with an intensity ranging from 55–70% of the maximum heart rate, which was determined by test on a bicycle ergometer. The effects of the exercise were monitored by means of the following parameters: maximum oxygen uptake (VO2peak), resting heart rate, systolic blood pressure and diastolic blood pressure. [Results] The results indicate statistically significant post-exercise improvements in heart rate, relative oxygen uptake and systolic blood pressure, among the members of the EXP group. The results indicate that at the initial measurement of cardiorespiratory fitness no statistically significant differences were found between the groups at the multivariate level (Wilk's λ=0.83), while statistically significant differences in the cardiorespiratory fitness were found at the final measurement (Wil's λ=0.430). [Conclusion] The obtained results indicate that the exercise program, which lasted for a period of 21 days, though shorter in duration than other programs still led to statistically significant changes in the CRF of individuals suffering from MI.

Background The effects of long-term exercise on steady-state dynamics of antioxidant defence system are not clear yet fully, and there is an evident lack of studies focused on combat sports. This study aimed to evaluate the parameters of oxidative stress in young judokas during four weeks pre-competition training period. Material/Methods: Changes of oxidative stress parameters (malondialdehyde, catalase, carbonyl and sulphydryl groups, and total antioxidant status) were studied during four week pre-competition training period on a sample consisting of 10 male judokas (age 20±1.3 years, sport experience 11±3.4 years). Results: The changes in all examined oxidative stress parameters showed no statistical significance. Results also suggested that well-developed anti-oxidant defence, particularly because of the superoxide dismutase activity, kept the amount of oxidized proteins unmodified. The explanation for this finding can be found in the facts that the subjects, although of young age, had a long training and competitive experience with the similar training programs and that they did not undergo rapid weight reduction. Conclusions: The obtained results suggest that four week pre-competition training period pattern had no effects on oxidative stress parameters in well-trained young judokas, and that natural antioxidant defences of the body responded adequately to complex training program.

In order to develop a reliable and rapid transgenic system for functional study of specific buckwheat gene constructs, two different in planta transformation methods were analyzed: vacuum infiltration and infiltration by syringe. The results indicated that the vacuum infiltration method was much more efficient and can therefore be considered the method of choice for buckwheat transformation.

Background: The relationship between different surrogates of insulin resistance and left ventricular geometry in obese children is still unclear. Objective: We sought to explore the relationship between commonly used measures of insulin sensitivity/resistance (homeostatic model assessment index, serum uric acid, and triglycerides to high-density lipoprotein cholesterol ratio) and left ventricular geometry in normotensive obese children. Methods: In this cross-sectional study, 32 normotensive obese children were examined. Transthoracic echocardiography was used to measure left ventricular mass index and relative wall thickness. Homeostasis model assessment index, serum uric acid level, and a ratio of triglycerides to high-density lipoprotein cholesterol were used as markers of the insulin resistance. Simple and partial correlation analyses (to control for the effects of body mass index) were conducted to explore relationship between studied variables and left ventricular mass index or relative wall thickness as outcome variables. Results: We found positive correlations between homeostasis model assessment index and relative wall thickness (r = 0.47, p = 0.03) which remained significant after controlling for the effect of body mass index, z-score (r = 0.48, p = 0.03). The cutoff level of homeostasis model assessment index with the optimum sensitivity (Sn) and specificity (Sp) derived from the receiver operating characteristic (ROC) curves for predicting concentric remodelling was ≥5.51 with Sn = 83.33 and Sp = 68.75. Conclusion: There is a positive relationship between homeostasis model assessment index and relative wall thickness of obese normotensive children which may help to distinguish at risk obese normotensive children for the development of concentric left ventricular remodelling.