The present study aimed to evaluate the effect of the most common antidepressants on aquatic protozoa. Spirostomum ambiguum was used as the model protozoan. The biological activity of four antidepressants, namely fluoxetine, sertraline, paroxetine, and mianserin, toward S. ambiguum was evaluated. Sertraline was found to be the most toxic drug with EC50 values of 0.2 to 0.7 mg/L. The toxicity of the antidepressants depended on the pH of the medium and was the highest in alkaline conditions. Sertraline was also the most bioaccumulating compound tested, followed by mianserin. Slow depuration was observed after transferring the protozoa from the drug solutions to a fresh medium, which indicated possible lysosomotropism of the tested antidepressants in the protozoa. The biotransformation products were identified using a high-resolution mass spectrometer after two days of incubation of the protozoa with the tested antidepressants. Four to six potential biotransformation products were observed in the aqueous phase, while no metabolites were detected in the protozoan cells. Because of the low abundance of metabolites in the medium, their structure was not determined.
Pharmaceuticals in the aquatic environment may be decomposed by abiotic and biotic factors. Photodegradation is the most investigated abiotic process, as it occurs in the natural environment and may be applied in wastewater treatment technology. Although pharmaceuticals are detected in effluents and surface water in a mixture, the photodegradation process is mainly evaluated with single compounds. The photodegradation of fluoxetine (FLU) and fluvoxamine (FLX) in the presence of diclofenac (DCF) and triclosan (TCS) was investigated with HPLC and bioassay. FLU did not degrade under UV-Vis irradiation in SunTest CPS+ either with or without the tested additives, although small amounts of desmethyl fluoxetine and 4-(trifluoromethyl)phenol were formed. In contrast, during irradiation, FLX isomerized to cis-FLX. This process was enhanced by DCF and TCS, but to a lesser degree than by humic acids. Thus, the presence and composition of the matrix should be considered in the environmental risk assessment of pharmaceuticals. As the toxicity of the tested solutions depended only on the concentration of the tested drugs, it was suggested that the biological activity of the photodegradation products was lower than that of the parent compounds.
This study evaluated the uptake of secondary nano- and small microparticles by the protozoan Spirostomum ambiguum, comparing edible (baker’s yeasts) and inedible (red latex) particles. Secondary nano- and microplastic particles were prepared from household materials made of four different polymers and served to the protozoans separately and as two-component mixtures in different proportions. The number and content of food vacuoles formed by the protozoan were analyzed using a digital microscope. The microscopic results showed that the protozoans ingested the secondary microplastic particles to a similar degree as the latex microspheres but to a lesser extent compared to the nutritional food—baker’s yeasts. At the microplastic concentrations of 1000 and 10,000 particles mL–1, no food vacuoles were observed inside the cells, which may be a finding of great ecological importance. In the protozoans served two-component mixtures, both microplastics and yeasts were found in the vacuoles formed by the organisms. The egestion of two-component vacuoles by the protozoans was slower than that of vacuoles containing a single component.
Antidepressants, especially selective serotonin re-uptake inhibitors, which are among the most commonly used pharmaceuticals, are ubiquitous in effluents and freshwaters. Microparticles, including microplastics, show sorption properties to different compounds, thus becoming a potential vector of toxic substances. This study aimed to evaluate the effects of four antidepressants on the protozoan Spirostomum ambiguum in the presence of four types of microplastics and baker’s yeast. The Spirotox, measuring the acute toxicity, and food uptake inhibition assay were applied. The microparticles did not influence the toxicity of the tested antidepressants in the acute toxicity assay. Moreover, they did not adsorb the drugs during a seven-day incubation in dark. However, sublethal levels of sertraline and duloxetine decreased the number of food vacuoles formed by the protozoa. The highest effect was observed in the case of the suspension of edible particles of baker’s yeast, where a significant decrease in the number of food vacuoles was observed in the sertraline concentration as low as 0.025 mg L−1. A lower but statistically significant effect was observed when wettable microparticles of phenolic resin were used as the artificial food source. These results indicate that serotonin re-uptake inhibitors can interfere with the feeding processes of ciliates.
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