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The reaction center-binding protein D1 of photosystem II (PS II) undergoes rapid turnover under light stress conditions. In the present study, we investigated the role of the extrinsic 33 kDa protein (OEC33) in the early stages of D1 turnover. D1 degradation was measured after strong illumination (1000-5000 microE m-2 S-1) of spinach manganese-depleted, PSII-enriched membrane and core samples in the presence and absence of the OEC33 under aerobic conditions at room temperature. PSII samples lacking the OEC33 were prepared by standard biochemical treatments with Tris or CaCl2/NH2OH while samples retaining the OEC33 were prepared with NH2OH or NaCl/NH2OH. The degradation of D1, monitored by SDS/urea-polyacrylamide gel electrophoresis and Western blotting using specific antibodies against D1, proceeds to a greater extent in NH2OH-treated samples than in Tris-treated samples over a 60 min illumination period. Under the same conditions, significantly more aggregation of D1 occurs in the Tris-treated samples than in the NH2OH-treated samples. The lower level of D1 degradation in Tris-treated samples is not due to secondary proteolysis, as judged from the time course for degradation at 25 degrees C or the degradation pattern at 4 degrees C. Similarly, for NaCl/NH2OH-treated samples, D1 degradation is greater and D1 aggregation less than in CaCl2/NH2OH-treated samples. The effect of the presence of the OEC33 on D1 degradation and aggregation is confirmed by reconstitution experiments in which the isolated OEC33 is restored back to Tris-treated samples. During very strong illumination, significant loss of CP43 also occurs in Tris-treated but not in NH2OH-treated samples. Structural analysis of PS II core complexes by Fourier transform infrared (FT-IR) spectroscopy revealed very little change in the protein secondary structure after 10 min illumination of NH2OH-treated samples while a large 10% decrease of alpha-helix content occurs in Tris-treated samples. On the basis of these results, we suggest that either (1) the OEC33 stabilizes the structural integrity of PS II such that it prevents the photodamaged D1 protein from aggregating with nearby polypeptides and thereby facilitating degradation or (2) the OEC33 specifically stabilizes CP43, a putative D1-specific protease, which normally promotes the efficient degradation of D1.

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Crop production under hydroponic environments has many advantages, yet the effects of solution flow rate on plant growth remain unclear. We conducted a hydroponic cultivation study using different flow rates under light-emitting diode lighting to investigate plant growth, nutrient uptake, and root morphology under different flow rates. Swiss chard plants were grown hydroponically under four nutrient solution flow rates (2 L/min, 4 L/min, 6 L/min, and 8 L/min). After 21 days, harvested plants were analyzed for root and shoot fresh weight, root and shoot dry weight, root morphology, and root cellulose and hemicellulose content. We found that suitable flow rates, acting as a eustress, gave the roots appropriate mechanical stimulation to promote root growth, absorb more nutrients, and increase overall plant growth. Conversely, excess flow rates acted as a distress that caused the roots to become compact and inhibited root surface area and root growth. Excess flow rate thereby resulted in a lower root surface area that translated to reduced nutrient ion absorption and poorer plant growth compared with plans cultured under a suitable flow rate. Our results indicate that regulating flow rate can regulate plant thigmomorphogenesis and nutrient uptake, ultimately affecting hydroponic crop quality.

Swiss chard (Beta vulgaris var. cicla L.) is a halophilic plant, which has increased growth under moderate salinity. The stimulation of total nitrogen (N) concentration was also observed with the increase of sodium chloride (NaCl) levels in previous studies. The purpose of this study was to investigate whether the stimulation of N uptake is the result of growth stimulation or the direct effect of NaCl. We compared the effects of NaCl on nitrate (NO 3 -) uptake and nitrate reductase (NR) activity in halophilic Swiss chard with those in salinity-tolerant barley (Hordeum vulgare L.). Both species were grown hydroponically at NaCl concentrations ranging from 0 to 100 mmol L −1 . Leaf dry weight of both species was not significantly affected by the treatments. In barley extracted leaf sap, the NO 3 -concentration was positively correlated with the potassium ion (K + ) concentration, but negatively correlated with the sodium ion (Na + ) concentration. In contrast, in Swiss chard extracted leaf sap, the Na + and NO 3 -concentrations were strongly and positively correlated. High salinity enhanced NR activity in leaves of Swiss chard, but decreased that in barley. Since NO 3-is a substrate of NR, increased NO 3 -uptake may explain the increased NR activity. We found that the effect of NaCl on NO 3 -uptake in halophilic Swiss chard and salinity-tolerant barley was completely different. We concluded that the NO 3 -uptake was enhanced directly by Na + in Swiss chard.