1 Selectins play an important role on leukocytes in®ltration into in¯ammatory tissues. To understand the role of selectins, we investigated the eects of selectin-IgG chimeras and anti selectin antibodies on the murine IgE-mediated skin in¯ammation model. 2 Biphasic skin reactions were induced by intradermal challenge with ovalbumin (OA) to ears of actively sensitized mice. This reaction was characterized by immediate and late phase responses observed as which were induced via a rapid increase in capillary permeability and leukocyte in®ltration, respectively. The expression of E-selectin mRNA was signi®cantly increased to reach its highest level at 2 h after OA challenge. 3 E-, P-, and L-selectin-IgG chimeras inhibited the late phase responses, i.e. ear swelling, neutrophil in®ltration and eosinophil in®ltration at 24 h after OA challenge in a dose-dependent manner at dose range of 0.1 ± 10 mg kg 71 , i.v. Antiselectin antibodies did not inhibit the increase of ear swelling. But anti E-and P-selectin antibodies signi®cantly inhibited neutrophil in®ltration and eosinophil in®ltration. 4 These results indicate that selectins play an important role on the late phase response of the murine IgE-mediated skin in¯ammation model by mediating in¯ammatory cell adhesion to endothelium.
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