Estudio comparativo de las complicaciones de la laparotomía subcostal mediante la técnica small bites frente a la large bitesComparative study of subcostal laparotomy complications using the small bites versus large bite technique
INTRODUCTION
Chemotherapy hinders liver function and probably its regenerating capacity, forcing to delay it after surgery. Our objective has been to verify this effect in an experimental model and to see if a hepatotrophic agent can prevent it.
MATERIAL AND METHODS
Four groups of 6 WAG/RijHsd rats (males, 3-4 months) were submitted to ligation of the portal branch to the left lateral and left paramedian lobes. They were sacrificed 36 h later to quantify the percentage of liver corresponding to the ligated lobes (weight), the number of hepatocyte’s nuclei (nº/100 µm2) and their mean size (µm2). One group received no treatment (control); another folic acid (2.5 mg/kg ip, during surgery); other 5-Fluorouracil (5-FU 50 mg/kg ip 48 h before); and the fourth received folic&5-FU.
RESULTS
The animals treated with folic acid showed a greater number of hepatocyte’s nuclei (24.4 ± 2.77 vs 15.2 ± 1.51) and their mean size was also greater (121 ± 2.34 vs 111 ± 1.8). However, the reduction in weight of the ligated parenchyma was less than in control group (33.4 ± 1.08 vs 29.5 ± 1.08). 5-FU did not modify the number of nuclei (15.6 ± 18.4), although they were smaller in size (104 ± 1.7). The addition of folic acid to 5-FU increased the number of nuclei (21.7 ± 2.8) and normalized their size (111 ± 3.2).
CONCLUSIONS
5-FU exerts a depressant effect on livers regeneration, and folic acid overcomes it. Thus, folic acid could allow early application of chemotherapy without affecting liver regeneration.
Conflictos de interés: los autores declaran no tener conflictos de interés.Financiaciones: los autores declaran no haber recibido apoyo financiero para la investigación, autoría y/o publicación de este artículo.
Evaluación de los resultados del cierre de una laparotomía media en cirugía de urgencias tras la introducción de la técnica de cierre fascial small bites Evaluation of the results of midline laparotomy closure in emergency surgery after the introduction of the fascial closure with small bites technique 10.20960/rhh.00435 01/10/2022 OR 435 Evaluación de los resultados del cierre de una laparotomía media en cirugía de urgencias tras la introducción de la técnica de cierre fascial small bites Evaluation of the results of midline laparotomy closure in emergency surgery after the introduction of the fascial closure with small bites technique
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