We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non‐coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three‐region options, (1) rpoC1, rpoB and 1matK or (2) rpoC1, matK and psbA‐trnH as viable markers for land plant barcoding.
A present-day climatic model is presented in which extended wet spells of near-decadal duration and dry spells of similar length are explained on the basis of surface and upper tropospheric circulation variations. Wet speIls are shown to be the result of increased tropical atmospheric disturbances and tropical-temperate interaction, and to be linked to variations in the Walker Circulation. Conversely, dry spells are shown to result from diminished tropical activity over southern Africa, equatorward movement of westerly storm tracks and temperate perturbations in the westerlies.The present-day analogue is compared to preliminary spatial reconstructions of the climate of southern Africa over the last twenty-five millennia and is shown to have wide applicability in the explanation of the late-Quaternary palaeoelimates of the subcontinent. In particular, it is argued that the Last Glacial Maximum was associated with northward-displaced circulation conditions similar to those of present-day dry spells over the summer rainfall region, whereas the extensive moist conditions that prevailed for several thousand years after 9000 BP were analogous to present-day wet spell conditions with little apparent displacement of major circulation features.
We estimate the global BOLD Systems database holds core DNA barcodes (rbcL + matK) for about 15% of land plant species and that comprehensive species coverage is still many decades away. Interim performance of the resource is compromised by variable sequence overlap and modest information content within each barcode. Our model predicts that the proportion of species-unique barcodes reduces as the database grows and that ‘false’ species-unique barcodes remain >5% until the database is almost complete. We conclude the current rbcL + matK barcode is unfit for purpose. Genome skimming and supplementary barcodes could improve diagnostic power but would slow new barcode acquisition. We therefore present two novel Next Generation Sequencing protocols (with freeware) capable of accurate, massively parallel de novo assembly of high quality DNA barcodes of >1400 bp. We explore how these capabilities could enhance species diagnosis in the coming decades.
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