Abstract:We investigated whether refined follicle stimulating hormone (FSH) with only a little contaminating LH can promote the responsiveness of rabbits to multiple-ovulation treatment. One group of female rabbits was stimulated with refined porcine FSH (pFSH), an FSH source with low LH activity, and another group was treated with pFSH. The mean number of eggs recovered from donors stimulated with refined pFSH (27 ± 3) was significantly greater (P<0.05) than that with pFSH (20 ± 2). Furthermore, the mean number of remaining follicles of donors stimulated with refined pFSH (19 ± 4) was significantly greater (P<0.05) than that with pFSH (12 ± 1). To decrease the number of remaining follicles in donors treated with refined pFSH, the dose of human chorionic gonadotropin (hCG) was increased from 75 to 150. However, there were no differences in the numbers of eggs and remaining follicles. The results of the present study suggest that refined pFSH with little contaminating LH promotes the responsiveness of rabbits to multiple-ovulation treatment compared with pFSH. Key words: follicle-stimulating hormone, multiple-ovulation increase the number of embryos. However, this multiple injection method is time and labor consuming, and also gives strong stress to donor rabbits. Recently, it has been reported that a single injection of FSH dissolved in polyvinylpyrrolidone (PVP) solution given to rabbits induced multiple ovulation and was a practical alternative to 6 times injection of FSH dissolved in saline [10]. However, the yield and quality of embryos raised after multiple ovulation are variable and unpredictable owing to variations in ovarian response.
Introdução: Durante as fases críticas do desenvolvimento embriológico, o organismo encontra-se num estado de vulnerabilidade a interferências externas. Assim, o presente estudo tem por objetivo avaliar os impactos da restrição proteica durante a lactação na morfologia renal da prole. Métodos: Utilizou-se ratos adultos da linhagem Wistar, que foram induzidos ao cruzamento. Considerou-se o nascimento como o dia 0 e as matrizes foram divididas em dois grupos (dietas normoproteica - NP - e hipoproteica - LP). No dia 21, os animais sofreram eutanásia e os rins foram preparados para análise histológica. Resultados: Evidenciou-se nos animais do grupo LP que o ganho de peso foi 63,33% menor, o número de glomérulos exibiu uma redução de 32,99% (P<0.0001), o comprimento glomerular apresentou uma diminuição de 12,70% (P<0.001) e a área total glomerular teve uma redução de 18,12% (P<0.01), quando comparados com o grupo NP. Conclusão: A restrição proteica materna durante o período de lactação provoca injúrias ao desenvolvimento renal da prole, evidenciadas pela menor contagem de glomérulos e menores dimensões das estruturas renais nos animais submetidos a essa dieta.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.