For the extraction process of the acid hydrolysis method, which is one of the main analytical methods for lipids, we developed a solid-phase extraction process using diatomaceous earth to replace the conventional liquidliquid extraction process, and we evaluated its analytical performance.In the analysis of lipids and fatty acid compositions, we obtained equivalent results between the conventional process and the newly developed process.In addition, this new process reduced the experimental time (person -hours) when compared to the conventional process.From these results, we consider the solid-phase extraction process to be a suitable and useful substitute for the conventional process in the acid hydrolysis method.
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