Citrus sinensis and Citrus limonia were obtained
by germination from seeds, and isotopic-labeling experiments using d-[1-13C]glucose were performed with the seedlings.
After 60 days, the seedlings were analyzed by high-performance liquid
chromatography–ultraviolet–solid-phase extraction–nuclear
magnetic resonance, data and the 13C enrichment patterns
of xanthyletin and seselin indicated that the pyran ring was formed
by the methylerythritol phosphate pathway and that the coumarin moiety
was derived from the shikimate pathway in both compounds. This information
regarding the biosynthetic pathway can be used to increase resistance
against phytopathogens, because xanthyletin and seselin are reported
to have antimicrobial activity on the growth of Xylella
fastidiosa, which causes citrus variegated chlorosis
in orange.
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