Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis. A real-time PCR assay was developed for the detection of B. anthracis. After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3' minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains. The assay was found to be specific for all 43 strains of B. anthracis tested. In addition, a test panel of 105 samples was analyzed to evaluate the potential diagnostic capability of the assay. The assay showed 100% specificity, demonstrating the usefulness of the gyrA gene as a specific chromosomal marker for B. anthracis.

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In southern Africa, there has been a long-standing but unsubstantiated assumption that the site of Khami evolved out of Great Zimbabwe's demise around ad 1450. The study of local ceramics from the two sites indicate that the respective ceramic traditions are clearly different across the entire sequence, pointing towards different cultural affiliations in their origins. Furthermore, there are tangible typological differences between and within their related dry-stone architecture. Finally, absolute and relative chronologies of the two sites suggest that Khami flourished as a major centre from the late fourteenth/early fifteenth century, long before Great Zimbabwe's decline. Great Zimbabwe also continued to be occupied into the late seventeenth and perhaps eighteenth centuries, after the decline of Khami. Consequently, the combined significance of these observations contradicts the parent-offspring relationship implied in traditional frameworks. Instead, as chronologically overlapping entities, the relationship between Khami and Great Zimbabwe, was heterarchical. However, within the individual polities, malleable hierarchies of control and situational heterarchies were a common feature. This is in tune with historically documented political relations in related pre-colonial southern Zambezian states, and motivates for contextual approaches to imagining power relations in pre-colonial African contexts.

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At the ancient Shona centre of Great Zimbabwe (1200–1700 CE), cattle (Bos taurus) were centrally important for economic, social, and symbolic purposes. 87Sr/86Sr for modern plants collected in southern Zimbabwe vary from 0.7054 to 0.8780 and ranges differ between some geological substrates. 87Sr/86Sr in serial samples of Bos taurus tooth enamel provides information on where animals consumed at Great Zimbabwe were raised and how herds were managed. The majority of animals sampled were born and remained for their first year of life in a region some 40–120 km south of Great Zimbabwe. Few animals came from geological substrates like that of Great Zimbabwe itself, and none from areas underlain by basalts (> 120 km south of Great Zimbabwe). Earlier hypotheses of transhumance are not supported. These findings will help to build a fuller picture of the role of local commodities (in this case, cattle) in the economic networks that supported the rise and florescence of Great Zimbabwe as a major centre of power.