no-bridgeKS49 (nob) has been shown to have a disturbed protocerebral bridge (pb). Using monoclonal antibodies that stain selectively some central complex regions we found that the protocerebral pridge defect is accompanied by a loss of certain inputs and outputs of the central complex. Mutant nob flies have a reduced climbing activity due to a reduced average walking speed and to a spastic state. Their mean longevity is drastically reduced, suggesting that nob is a pleiotropic gene. The nob mutant is impaired in habituation and in Pavlovian conditioning of the proboscis extension reflex (PER), although its initial reactivity to the sucrose stimulus is normal. Tested in habituation of the PER by ipsi- and contralateral stimulations, the nob mutant shows some difficulties in the "data exchange" between the two brain hemispheres, suggesting that the central complex may coordinate information processing in the two hemispheres.
In insects the level of serotonin can be modified through the alimentary tract. Drosophila fed on chemically defined media to which has been added a synthesis inhibitor or an immediate precursor of this neurotransmitter have their serotonin level decreased or increased, respectively. (A 4- to 5-day treatment is optimal). Such treated flies, if tested in a procedure of Pavlovian conditioning, have different learning performances. With the same duration of starvation, nearly the same threshold for sucrose solutions, but different amounts of serotonin, the flies (blindly trained) showed conditioned inhibition of the tarsal (or proboscis-extension) reflex according to the amount of serotonin. The highest level of this biogenic amine gave the best performance.
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